Document 1076
 DOCN  M9591076
 TI    Synthetic peptides representing sequences within gp41 of HIV as
       immunogens for murine T- and B-cell responses.
 DT    9509
 AU    Brown LE; White DO; Agius C; Kemp BE; Yatzakis N; Poumbourios P; McPhee
       DA; Jackson DC; Department of Microbiology, University of Melbourne,
       Parkville,; Victoria, Australia.
 SO    Arch Virol. 1995;140(4):635-54. Unique Identifier : AIDSLINE
       MED/95314456
 AB    Within the gp41 glycoprotein of the human immunodeficiency virus type 1
       (HIV-1) there is a relatively conserved region which appears accessible
       to the immune system during the course of HIV infection and is
       recognised by antibody from virtually all patients with AIDS. This
       region has also been shown to function as a target for human T cells. We
       have examined synthetic peptides spanning this sequence, between
       residues 572 and 604, with a view to evaluating their potential as
       immunogens. Peptides 572GIKQLQARILAVERYLKDQQ591 and
       579RILAVERYLKDQQLLGGIWGCSGK601 were good immunogens in two different
       strains of mice while peptide 576LQARILAVERYLKDQQ591 was an inferior
       immunogen, and peptide 593LGIWGCSGKLIC604 was non-immunogenic unless
       coupled to a carrier protein. For both antibody and T cell responses it
       was apparent that sequences that could function as determinants within
       one peptide could not do so in the context of a different peptide
       immunogen. It follows that by judicious choice of immunogen sequence it
       may be possible to direct the immune response towards a desired fine
       specificity. Unwanted responses by CD4+ T cells isolated from certain
       peptide-primed animals were also observed. These T cells showed an
       unusual reactivity in that they were incapable of recognising their
       determinant AVERYLKDQQ if it was extended at the C-terminal end with the
       native sequence and as such would not be expected to recognise the
       native molecule unless processing created the identical C-terminus.
 DE    Amino Acid Sequence  Animal  Antigenic Determinants/IMMUNOLOGY
       B-Lymphocytes/*IMMUNOLOGY  Cloning, Molecular  Female  HIV
       Antigens/IMMUNOLOGY  HIV Envelope Protein gp41/GENETICS/*IMMUNOLOGY
       Mice  Mice, Inbred BALB C  Mice, Inbred CBA  Mice, Inbred C57BL
       Molecular Sequence Data  Peptide Fragments/CHEMICAL
       SYNTHESIS/GENETICS/IMMUNOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes/*IMMUNOLOGY  T-Lymphocytes, Helper-Inducer/IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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