Document 1083
 DOCN  M9591083
 TI    French multicenter study involving eight test sites for radiometric
       determination of activities of 10 antimicrobial agents against
       Mycobacterium avium complex.
 DT    9509
 AU    Rastogi N; Bauriaud RM; Bourgoin A; Carbonnelle B; Chippaux C; Gevaudan
       MJ; Goh KS; Moinard D; Roos P; Unite de la Tuberculose & des
       Mycobacteries, Institut Pasteur,; Guadeloupe, French West Indies.
 SO    Antimicrob Agents Chemother. 1995 Mar;39(3):638-44. Unique Identifier :
       AIDSLINE MED/95314195
 AB    The radiometric BACTEC 460-TB methodology has filled an increased need
       in the screening of a wide range of antimicrobial agents against
       Mycobacterium avium (MAC) isolates on a patient-to-patient basis. In
       this context, a multicenter study involving eight test sites across
       France was performed to determine the MICs of 10 antimicrobial agents
       for MAC organisms. The aim of the investigation was to compare the in
       vitro activities of D-cycloserine, ethambutol, ethionamide, rifampin,
       amikacin, streptomycin, ciprofloxacin, sparfloxacin, clofazimine, and
       clarithromycin against MAC isolates. All of the test sites received the
       same clinical isolates of MAC, and the MICs were determined by a common
       protocol. The overall interlaboratory reproducibility of the MICs within
       +/- 1 dilution of the modal MICs varied from 79.70 to 100% (mean, 95.2%
       +/- 2.1%), whereas overall agreement of the MICs among the test sites
       varied from a mean of 91% +/- 4.1% to a mean of 98 +/- 1.3%. We
       confirmed that the proposed methodology is easy, accurate, and
       sufficiently reproducible to be used routinely in a clinical laboratory.
       Despite variations in the MICs of the same drug among strains, no link
       between the origin of MAC isolates (from human immunodeficiency
       virus-positive or -negative patients) and their drug susceptibilities
       was established. On the basis of the MICs that inhibited 50 and 90% of
       isolates tested for the drugs used, clarithromycin, clofazimine,
       ethambutol, and streptomycin were the most uniformly active against MAC;
       this was followed by amikacin, rifampin, and sparfloxacin. On the other
       hand, ciprofloxacin, D-cycloserine, and ethionamide showed only marginal
       in vitro activities.
 DE    Anti-Infective Agents/*PHARMACOLOGY  Comparative Study  France  Human
       HIV Infections/COMPLICATIONS  Microbial Sensitivity Tests  Mycobacterium
       avium Complex/*DRUG EFFECTS  Mycobacterium avium-intracellulare
       Infection/MICROBIOLOGY  Radiometry  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE  MULTICENTER STUDY

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