Document 1113
 DOCN  M9591113
 TI    Bone marrow one step fixation-decalcification in Lowy FMA solution: an
       immunohistological and in situ hybridization study.
 DT    9509
 AU    Gaulier A; Fourcade C; Szekeres G; Pulik M; Service d'Anatomie Cytologie
       Pathologiques, C. H. Victor Dupouy,; Argenteuil, France.
 SO    Pathol Res Pract. 1994 Dec;190(12):1149-61. Unique Identifier : AIDSLINE
       MED/95312436
 AB    The immunoreactivity of paraffin embedded bone marrow biopsies (BMB) was
       studied following a one step 20-hour-fixation-decalcification in Lowy
       formalin mercuric chlorid acid solution which permits excellent
       histological stainings. Antibodies reactive with myeloid,
       megakaryocytic, erythroid cells, T and B lymphocytes, mastocytes and
       metastatic cells were compared. Nearly all antibodies working on
       paraffin sections were demonstrated on Lowy FMA fixed BMB. Special care
       was taken to define an optimal working dilution. Trypsinization was not
       necessary. A slide microwave pre-treatment appeared essential before
       testing CD20 L26, CD8, CD3, CD34, MB1 Kappa and Lambda antibodies. It
       was suitable for UCHL1, LN2, CD30 antibodies. The same fixative allowed
       an m RNA Kappa or Lambda in myeloma and EBER 1 EBV RNAs in HIV lymphoma
       visualization by in situ hybridization. The safety handling of the toxic
       mercuric chloride component is discussed.
 DE    Biopsy  Bone Marrow/*PATHOLOGY  *Fixatives  *Formaldehyde  Histological
       Techniques  Human  Immunohistochemistry  In Situ Hybridization
       *Mercuric Chloride  Paraffin Embedding  Stains and Staining  JOURNAL
       ARTICLE

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