Document 1141
 DOCN  M9591141
 TI    Human immunodeficiency virus type-1 can be detected in monocytes by
       polymerase chain reaction.
 DT    9509
 AU    Quiros E; Garcia F; Maroto MC; Bernal MC; Cabezas T; Piedrola G;
       Department of Microbiology, Faculty of Medicine, University of; Granada,
       Spain.
 SO    J Med Microbiol. 1995 Jun;42(6):411-4. Unique Identifier : AIDSLINE
       MED/95311302
 AB    Lymphocytes and monocytes from 25 patients infected with human
       immunodeficiency virus type-1 (HIV-1)--13 asymptomatic, seven with the
       AIDS-related complex (ARC) and five with the acquired immunodeficiency
       syndrome (AIDS)--were lysed and subjected to PCR with three primer
       pairs: SK38/SK39 (gag), SK68/SK69 (env) and SK29/SK30 (LTR). Amplified
       DNA was solution-hybridised with 32P-labelled probes (SK19, SK70 and
       SK31, respectively) and detected by PAGE-autoradiography. HIV-1 DNA was
       detected as follows. Asymptomatic patients: monocytes--gag 61.5%, env
       100%, LTR 0%; lymphocytes--gag 100%, env 92.3%, LTR 53.84%. ARC
       patients: monocytes--gag 71.4%, env 57.1%, LTR 0%; lymphocytes--gag
       100%, env 71.4%, LTR 71.4%. AIDS patients: monocytes--gag 80.0%, env
       100%, LTR 0%; lymphocytes--gag 100%, env 60%, LTR 60%. The presence of
       HIV-1 DNA was confirmed in the monocyte fraction. In this cell subset,
       the env gene-directed primers were the most effective for amplification,
       whereas the LTR gene-directed primers failed to amplify HIV-1 DNA. The
       different pattern of amplification found in monocytes may suggest that
       these cells could be infected by a genetic variant of the virus.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/VIROLOGY  AIDS-Related
       Complex/BLOOD/VIROLOGY  Carrier State/BLOOD/VIROLOGY  DNA, Viral/*BLOOD
       Genes, env  Genes, gag  Genes, Viral  Human  HIV
       Infections/*BLOOD/VIROLOGY  HIV-1/GENETICS/*ISOLATION & PURIF
       Lymphocytes/VIROLOGY  Monocytes/*VIROLOGY  Polymerase Chain Reaction
       Repetitive Sequences, Nucleic Acid/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).