Document 1142
 DOCN  M9591142
 TI    Evidence of Pneumocystis carinii in cell line cultures infected with
       peripheral blood mononuclear cells isolated from AIDS patients with P.
       carinii pneumonia.
 DT    9509
 AU    Contini C; Mastrantoni S; Romani R; Cultrera R; Delia S; Institute of
       Infectious and Respiratory Diseases, University of; Ferrara, Italy.
 SO    J Med Microbiol. 1995 Jun;42(6):394-8. Unique Identifier : AIDSLINE
       MED/95311299
 AB    The detection of Pneumocystis carinii was investigated in an in-vitro
       system consisting of a human lung epithelial cell line (A-549)
       inoculated with infected peripheral blood mononuclear cells (PMBC) from
       HIV-infected patients with proven or suspected P. carinii pneumonia
       (PCP), and from HIV-negative patients with other lung infections.
       Supernates from cultures were sampled daily and evaluated for the
       presence of P. carinii by Giemsa and immunofluorescence staining. P.
       carinii was isolated from 98 (95.1%) of 103 culture supernate samples
       from patients with proven pneumocystosis and 45 (66.1%) of 68 from
       patients with suspected PCP 40 or 72 h after PBMC inoculation. This
       system has been shown to support the growth of P. carinii but did not
       seem to be adequate for the production of large numbers of organisms,
       although long-term survival in vitro for up to 3 weeks was observed.
       Recovery of P. carinii from infected PBMC strongly supports previous
       observations about its ability to disseminate haematogenously and could
       represent a further advance in understanding the pathogenesis and
       diagnosis of PCP.
 DE    Adult  AIDS-Related Opportunistic Infections/*BLOOD/DIAGNOSIS/
       MICROBIOLOGY  Cell Line  Epithelium/CYTOLOGY/MICROBIOLOGY  Female
       Fluorescent Antibody Technique  Human  Leukocytes,
       Mononuclear/*MICROBIOLOGY  Lung/CYTOLOGY/MICROBIOLOGY  Male  Middle Age
       Pneumocystis carinii/*ISOLATION & PURIF  Pneumonia, Pneumocystis
       carinii/*BLOOD/DIAGNOSIS/MICROBIOLOGY  JOURNAL ARTICLE

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