Document 1145
 DOCN  M9591145
 TI    Nocodazole-dependent transport, and brefeldin A--sensitive processing
       and sorting, of newly synthesized membrane proteins in cultured neurons.
 DT    9509
 AU    Cid-Arregui A; Parton RG; Simons K; Dotti CG; Cell Biology Program,
       European Molecular Biology Laboratory,; Heidelberg, Germany.
 SO    J Neurosci. 1995 Jun;15(6):4259-69. Unique Identifier : AIDSLINE
       MED/95310983
 AB    The envelope glycoproteins of Semliki Forest virus (SFV), Vesicular
       Stomatitis virus (VSV), and Influenza Fowl Plague virus (FPV) are
       vectorially targeted in neurons to the plasma membrane of dendrites (SFV
       and VSV) and axons (FPV). To gain insight into the mechanisms
       responsible for such polarized delivery we have examined the effects on
       neurons of nocodazole and brefeldin A (BFA), which are known to cause
       microtubule depolymerization and disassembly of the Golgi apparatus,
       respectively. Nocodazole treatment blocked transport of all viral
       glycoproteins to both axons and dendrites. BFA treatment induced
       disruption of the Golgi complex, including the trans-Golgi network
       (TGN), and tubulation of endosomes. However, the delivery of the SFV and
       FPV glycoproteins to the cell surface was not affected significantly by
       BFA, although processing and sorting were altered, as revealed by
       surface biotinylation and immunofluorescence microscopy of fixed
       nonpermeabilized cells. These results demonstrate the involvement of
       microtubules in axonal and dendritic transport of integral membrane
       glycoproteins, and the existence of a BFA-sensitive component in the
       sorting but not in the transport machinery.
 DE    Animal  Cells, Cultured  Cyclopentanes/*PHARMACOLOGY  Fetus  Fluorescent
       Antibody Technique  Gene Products, env/*BIOSYNTHESIS  Golgi
       Apparatus/DRUG EFFECTS/METABOLISM/ULTRASTRUCTURE  Hippocampus/METABOLISM
       Kinetics  Membrane Proteins/*BIOSYNTHESIS  Methionine/METABOLISM
       Microscopy, Electron  Microtubules/DRUG
       EFFECTS/METABOLISM/ULTRASTRUCTURE  Neurons/DRUG EFFECTS/*METABOLISM
       Nocodazole/*PHARMACOLOGY  Orthomyxovirus Type A, Avian/METABOLISM
       Protein Processing, Post-Translational/*DRUG EFFECTS  Protein Synthesis
       Inhibitors/*PHARMACOLOGY  Rats  Rats, Sprague-Dawley  Semliki Forest
       Virus/METABOLISM  Sulfur Radioisotopes  Support, Non-U.S. Gov't
       Vesicular Stomatitis-Indiana Virus/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).