Document 1149
 DOCN  M9591149
 TI    T cell receptor V beta repertoire in an acute infection of rhesus
       monkeys with simian immunodeficiency viruses and a chimeric simian-human
       immunodeficiency virus.
 DT    9509
 AU    Chen ZW; Kou ZC; Lekutis C; Shen L; Zhou D; Halloran M; Li J; Sodroski
       J; Lee-Parritz D; Letvin NL; Harvard Medical School, Beth Israel
       Hospital, Boston,; Massachusetts 02215, USA.
 SO    J Exp Med. 1995 Jul 1;182(1):21-31. Unique Identifier : AIDSLINE
       MED/95310853
 AB    Changes in T cell receptor (TCR) V beta repertoire and their correlation
       with virologic events were investigated in rhesus monkeys after acute
       infection with the simian immunodeficiency virus (SIV). 11 genetically
       defined rhesus monkeys were experimentally infected with SIVmac or a
       chimeric simian-human immunodeficiency virus (SHIV), and their
       peripheral blood lymphocytes (PBL) and lymph nodes were prospectively
       assessed for TCR V beta gene expression. PBL and lymph nodes of the
       acutely infected monkeys demonstrated an expansion of selected V
       beta-expressing T lymphocyte subpopulations as early as 3 d after
       infection. These expanded V beta-expressing lymphocyte subpopulations
       were comprised predominantly of CD8+ cells. Six of seven infected
       monkeys sharing a single electrophoretically defined major
       histocompatibility complex class I allele exhibited a similar expansion
       of V beta 14-expressing PBL. Sequence analyses of V-D-J segments of
       TCR-beta cDNA indicated that the V beta-expressing T cell subpopulation
       expansion can be oligoclonal. SIVmac-specific CD8+ cytotoxic T
       lymphocytes were demonstrated in both PBL and lymph nodes of the
       infected monkeys at the time expansion of the selected V beta-expressing
       cell subpopulations was seen. Finally, the expansion of the selected V
       beta-expressing lymphocytes in PBL coincided with the emergence and
       clearance of SIV p27 from the plasma of the infected monkeys. These
       results demonstrate that acute infection of rhesus monkeys with SIVmac
       or SHIV results in an expansion of CD8+ lymphocyte subpopulations
       expressing selected V beta gene families. The selectively expanded T
       lymphocytes may contribute to early viral clearance after acute SIVmac
       or SHIV infection.
 DE    Acute Disease  Amino Acid Sequence  Animal  Base Sequence  Comparative
       Study  *CD8-Positive T-Lymphocytes  DNA, Complementary/GENETICS  *Gene
       Expression Regulation, Viral  *Gene Rearrangement, beta-Chain T-Cell
       Antigen Receptor  Genes, MHC Class I  Genes, MHC Class II
       HIV-1/*GENETICS  Macaca mulatta  Molecular Sequence Data  Receptors,
       Antigen, T-Cell, alpha-beta/*GENETICS  RNA/GENETICS  RNA, Viral/GENETICS
       Simian Acquired Immunodeficiency Syndrome/*IMMUNOLOGY  Support, U.S.
       Gov't, P.H.S.  SIV/*GENETICS  *T-Lymphocyte Subsets  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).