Document 1150
 DOCN  M9591150
 TI    Human immunodeficiency virus type 1 neutralization is determined by
       epitope exposure on the gp120 oligomer.
 DT    9509
 AU    Sattentau QJ; Moore JP; Centre d'Immunologie de Marseille Luminy,
       Marseille, France.
 SO    J Exp Med. 1995 Jul 1;182(1):185-96. Unique Identifier : AIDSLINE
       MED/95310850
 AB    The major target of the neutralizing antibody response to infection by
       the human immunodeficiency virus type 1 (HIV-1) is the outer envelope
       glycoprotein, gp120. The spectrum of HIV-1 neutralization specificity is
       currently represented by monoclonal antibodies (mAbs) that can be
       divided broadly into five groups. We have studied the binding of these
       mAbs to functional oligomeric and soluble monomeric gp120 derived from
       the molecular clone of a cell line-adapted isolate of HIV-1, and
       compared these binding properties with virus neutralization. Binding of
       all mAbs except those reactive with the V3 loop was much weaker to
       oligomeric than to monomeric gp120. This reduction in binding to
       oligomeric gp120 was determined mostly by a slower relative rate of
       association, although the dissociation rate also had some influence on
       relative variation in mAb affinity. Virus neutralization correlated
       broadly with mAb binding to the oligomeric rather than to the monomeric
       form of gp120, and neutralization potency was related to the estimated
       association rate. Thus, with the exception of the hypervariable V3 loop,
       regions of HIV-1 gp120 with the potential to induce a neutralization
       response are likely to be poorly presented for antibody recognition on
       the surface of cell line-adapted virions.
 DE    Antibodies, Monoclonal/*IMMUNOLOGY/METABOLISM  Antibody Affinity
       Antigen-Antibody Reactions  Antigenic
       Determinants/*IMMUNOLOGY/METABOLISM  Antigens, CD4/METABOLISM  Cell Line
       Comparative Study  Human  HIV Antibodies/*IMMUNOLOGY/METABOLISM  HIV
       Envelope Protein gp120/*IMMUNOLOGY/METABOLISM  HIV-1/*IMMUNOLOGY
       Neutralization Tests  Peptide Fragments/IMMUNOLOGY/METABOLISM  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Virion/IMMUNOLOGY  JOURNAL
       ARTICLE

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