Document 1155 DOCN M9591155 TI Parasitic culture of buffy coat for diagnosis of visceral leishmaniasis in human immunodeficiency virus-infected patients. DT 9509 AU Lopez-Velez R; Laguna F; Alvar J; Perez-Molina JA; Molina R; Martinez P; Villarrubia J; Tropical Medicine and Clinical Parasitology Unit, Hospital Ramon; y Cajal, Madrid, Spain. SO J Clin Microbiol. 1995 Apr;33(4):937-9. Unique Identifier : AIDSLINE MED/95310468 AB Two samples of buffy coat from the peripheral blood of 25 human immunodeficiency virus-positive patients with proven visceral leishmaniasis, as determined with a bone marrow aspirate (stain and culture), were cultured onto Schneider's and Novy-McNeal-Nicolle media. Hemoculture positivity was 67%. The average growing time was 10 days. This is an easy, noninvasive, and sensitive technique. DE Animal AIDS-Related Opportunistic Infections/*COMPLICATIONS/*DIAGNOSIS/ PARASITOLOGY Bone Marrow/PARASITOLOGY Female Human Leishmania/*ISOLATION & PURIF Leishmaniasis, Visceral/*COMPLICATIONS/*DIAGNOSIS/PARASITOLOGY Male Parasitemia/*COMPLICATIONS/*DIAGNOSIS/PARASITOLOGY Parasitology/*METHODS/STATISTICS & NUMER DATA Phlebotomus Sensitivity and Specificity CLINICAL TRIAL JOURNAL ARTICLE SOURCE: National Library of Medicine. NOTICE: This material may be protected by Copyright Law (Title 17, U.S.Code).