Document 1156
 DOCN  M9591156
 TI    In vitro culture and serologic and molecular identification of Septata
       intestinalis isolated from urine of a patient with AIDS.
 DT    9509
 AU    Visvesvara GS; da Silva AJ; Croppo GP; Pieniazek NJ; Leitch GJ; Ferguson
       D; de Moura H; Wallace S; Slemenda SB; Tyrrell I; et al; Division of
       Parasitic Diseases, Centers for Disease Control and; Prevention,
       Atlanta, Georgia, 30341-3724, USA.
 SO    J Clin Microbiol. 1995 Apr;33(4):930-6. Unique Identifier : AIDSLINE
       GENBANK/U09929
 AB    Microsporidian spores were identified, on the basis of Weber's staining,
       in urine, stool, nasal, and saliva samples of an AIDS patient with
       diarrhea, hematuria, dysuria, and dementia. Urine and stool samples
       contained numerous spores, whereas few spores were seen in the nasal and
       saliva samples. Spores were concentrated from urine samples and
       inoculated into monkey kidney cell (E6) monolayers. After 6 to 8 weeks
       of culture, infected E6 cells filled with spores as well as spores free
       in the culture supernatants were seen daily. Transmission electron
       microscopy revealed that all stages of the parasite (CDC:V297) developed
       within septated, honeycomb-shaped parasitophorous vacuoles. Indirect
       immunofluorescence and immunoblotting studies using rabbit
       anti-Encephalitozoon cuniculi, anti-Encephalitozoon hellem, and
       anti-CDC:V297 sera revealed that CDC:V297 reacted intensely with the
       homologous serum but minimally with the heterologous sera. DNA isolated
       from CDC:V297, when PCR amplified with E. hellem and E. cuniculi
       primers, did not produce the diagnostic bands of approximately 547 and
       approximately 549 bp characteristic of E. hellem and E. cuniculi,
       respectively. On the basis of these studies, we concluded that CDC:V297
       fits the description of Septata intestinalis (A. Cali, D. P. Kotler, and
       J. M. Orenstein, J. Eukaryot, Microbiol. 40:101-112, 1993).
 DE    Adult  Animal  Antibodies, Protozoan  AIDS-Related Opportunistic
       Infections/*COMPLICATIONS/IMMUNOLOGY/  *PARASITOLOGY  Base Sequence
       Case Report  Cell Line  Cloning, Molecular  DNA Primers/GENETICS  DNA,
       Protozoan/GENETICS  Feces/PARASITOLOGY  Human  Male  Microscopy,
       Electron  Microspora Infections/*COMPLICATIONS/IMMUNOLOGY/*PARASITOLOGY
       Microsporida/CLASSIFICATION/GENETICS/*ISOLATION & PURIF  Molecular
       Sequence Data  Polymerase Chain Reaction  Protozoan
       Proteins/IMMUNOLOGY/ISOLATION & PURIF  Rabbits  Spores/ISOLATION & PURIF
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Urine/PARASITOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).