Document 1157
 DOCN  M9591157
 TI    Use of a colorimetric system for yeast susceptibility testing.
 DT    9509
 AU    Tiballi RN; He X; Zarins LT; Revankar SG; Kauffman CA; Department of
       Internal Medicine, Veterans Affairs Medical Center,; Ann Arbor,
       Michigan, USA.
 SO    J Clin Microbiol. 1995 Apr;33(4):915-7. Unique Identifier : AIDSLINE
       MED/95310464
 AB    We examined the reliability and accuracy of a colorimetric assay using
       Alamar Blue reagent in the performance of susceptibility tests for
       Candida albicans. We compared the broth macrodilution method recommended
       by the National Committee for Clinical Laboratory Standards (NCCLS) with
       a macrodilution method modified with the Alamar reagent and a
       microdilution method modified with the Alamar reagent. The MICs of
       fluconazole and itraconazole for 97 isolates of C. albicans and 3
       control isolates were tested. For fluconazole, the Alamar-modified broth
       macrodilution method yielded 94% (91 of 97) concordance within 2
       dilutions compared with the NCCLS method, while the microdilution method
       yielded 95% (92 of 97) concordance. With Alamar-modified methods for
       itraconazole, broth macrodilution yielded 97% (94 of 97) concordance
       within 2 dilutions. MICs obtained by the microdilution method, although
       tightly nested, were shifted to a higher value when compared with those
       obtained by the NCCLS method; there was only 77% (75 of 97) concordance
       within 2 dilutions but 97% concordance (94 of 97) within 3 dilutions.
       Tests by all methods with quality control strains showed excellent
       reproducibilities. For fluconazole, the methods modified with the Alamar
       reagent yielded clear endpoints and excellent correlation for the broth
       macrodilution and microdilution methods. For itraconazole, the methods
       modified with the Alamar reagent yielded clear endpoints and were
       reproducible, but higher MICs were obtained by the microdilution methods
       compared with those obtained by the NCCLS methods.
 DE    AIDS-Related Opportunistic Infections/COMPLICATIONS/MICROBIOLOGY
       Candida albicans/*DRUG EFFECTS/ISOLATION & PURIF  Candidiasis,
       Oral/COMPLICATIONS/MICROBIOLOGY  Colorimetry/*METHODS/STATISTICS & NUMER
       DATA/STANDARDS  Comparative Study  Dyes  Evaluation Studies
       Fluconazole/ADMINISTRATION & DOSAGE/PHARMACOLOGY  Human
       Itraconazole/ADMINISTRATION & DOSAGE/PHARMACOLOGY  Microbial Sensitivity
       Tests/*METHODS/STATISTICS & NUMER DATA/  STANDARDS
       Mycology/METHODS/STATISTICS & NUMER DATA/STANDARDS  Quality Control
       Reference Standards  Reproducibility of Results  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

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