Document 1158
 DOCN  M9591158
 TI    Simple determination of human immunodeficiency virus type 1
       syncytium-inducing V3 genotype by PCR.
 DT    9509
 AU    Fouchier RA; Brouwer M; Broersen SM; Schuitemaker H; Department of
       Clinical Viro-Immunology, Central Laboratory of The; Netherlands Red
       Cross Blood Transfusion Service, Amsterdam.
 SO    J Clin Microbiol. 1995 Apr;33(4):906-11. Unique Identifier : AIDSLINE
       MED/95310462
 AB    Human immunodeficiency virus type 1 (HIV-1) phenotype variability plays
       an important role in the pathogenesis of AIDS. The presence of
       syncytium-inducing (SI) HIV-1 isolates in infected individuals is
       associated with a rapid decline of CD4+ T cells, rapid disease
       progression, and reduced survival time after AIDS diagnosis. The strong
       association between the SI capacity of HIV-1 and the presence of
       positively charged amino acid residues at positions 306 and/or 320 in
       the third variable domain (V3) of gp120 could here be confirmed in 97%
       of 402 primary HIV-1 isolates, indicating that the V3 genotype may be
       useful for prediction of the viral phenotype. The V3 DNA sequences
       revealed a remarkably limited codon usage for the amino acid residues
       that are responsible for virus phenotype. On the basis of this limited
       SI-specific DNA sequence variation, four SI-specific oligonucleotides
       were designed for selective amplification of V3 from SI but not non-SI
       HIV-1 isolates. This PCR analysis allowed the prediction of the
       biological phenotype of HIV-1 isolates on the basis of the V3 genotype
       and may prove to be useful for monitoring SI capacity of HIV-1 isolates
       in infected individuals.
 DE    Amino Acid Sequence  Base Sequence  Cytopathogenic Effect,
       Viral/GENETICS  DNA Primers/GENETICS  DNA, Viral/GENETICS  Genotype
       Human  HIV Envelope Protein gp120/*GENETICS  HIV Infections/VIROLOGY
       HIV-1/*GENETICS/ISOLATION & PURIF/*PATHOGENICITY  Molecular Sequence
       Data  Peptide Fragments/*GENETICS  Phenotype  Polymerase Chain
       Reaction/*METHODS/STATISTICS & NUMER DATA  Sensitivity and Specificity
       Sequence Homology, Amino Acid  Support, Non-U.S. Gov't  Variation
       (Genetics)  Virology/METHODS/STATISTICS & NUMER DATA  JOURNAL ARTICLE

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