Document 1159
 DOCN  M9591159
 TI    Evaluation of risk and diagnostic value of quantitative assays for
       anti-Toxoplasma gondii immunoglobulin A (IgA), IgE, and IgM and
       analytical study of specific IgG in immunodeficient patients.
 DT    9509
 AU    Pinon JM; Foudrinier F; Mougeot G; Marx C; Aubert D; Toupance O; Niel G;
       Danis M; Camerlynck P; Remy G; et al; Laboratoire de Parasitologie, Eq 5
       Institut National de la Sante; et de la Recherche Medicale (Unite 314),
       Reims, France.
 SO    J Clin Microbiol. 1995 Apr;33(4):878-84. Unique Identifier : AIDSLINE
       MED/95310456
 AB    To determine their prognostic and diagnostic values for toxoplasmosis in
       immunodepressed subjects, we assayed immunoglobulin A (IgA) and IgE
       antibodies by means of immunocapture (IC) tests, with revelation done by
       using a suspension of T. gondii (ICT). We also carried out a
       simultaneous analytical study of IgG antibodies on cellulose acetate
       membranes by using the comparative immunological profile method and an
       enzyme-linked immunofiltration assay (ELIFA). A total of 1,238 samples
       (serum, cerebrospinal fluid, and aqueous humor from 318 patients) were
       tested. IgA and IgE antibodies were detected in all heart, kidney, and
       liver transplant recipients with clinical manifestations of
       toxoplasmosis; IgA was detected in the aqueous humor of a patient with
       chorioretinitis. In patients with AIDS-related toxoplasmosis, including
       the cerebral form, IgA and IgE antibodies or a significant modification
       of ELIFA IgG values were observed in 38, 19, and 25% of patients,
       respectively. IgM was detected by ICT only in 12% of patients and aided
       the diagnosis in 1 of 71 patients. IC tests for specific IgA and IgE
       alone and combined with ELIFA were positive in 39 and 46% of patients
       who developed clinical toxoplasmosis, respectively. In a serial study of
       16 patients in whom at least one of these three tests was positive, a
       significant immunological signal sometimes preceded clinical onset by 1,
       6, and even 17 months. Similarly, in a group of human immunodeficiency
       virus-infected patients with evidence of previous exposure to T. gondii
       but no clinical manifestations, IgA, IgE, and IgA and/or IgE antibodies
       were detected in only 11, 4, and 12% of patients, respectively.(ABSTRACT
       TRUNCATED AT 250 WORDS)
 DE    Adolescence  Adult  Animal  Antibodies, Protozoan/*ANALYSIS  Antibody
       Specificity  AIDS-Related Opportunistic
       Infections/COMPLICATIONS/DIAGNOSIS/  IMMUNOLOGY  Evaluation Studies
       Human  IgA/ANALYSIS  IgE/ANALYSIS  IgG/ANALYSIS  IgM/ANALYSIS
       Immunoassay/METHODS/STATISTICS & NUMER DATA  Immunocompromised Host
       Middle Age  Risk Factors  Sensitivity and Specificity  Support, Non-U.S.
       Gov't  Toxoplasma/*IMMUNOLOGY
       Toxoplasmosis/COMPLICATIONS/*DIAGNOSIS/*IMMUNOLOGY  Transplantation
       Immunology  JOURNAL ARTICLE

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