Document 1174
 DOCN  M9591174
 TI    Requirement of CD4+ T cells and antigen-presenting cells for primary in
       vitro generation of CD8+ cytotoxic T cells against Ld-binding
       self-peptide p2Ca.
 DT    9509
 AU    Wada H; Ono T; Uenaka A; Monden M; Nakayama E; Department of
       Parasitology and Immunology, Okayama University; Medical School, Japan.
 SO    Immunology. 1995 Apr;84(4):633-7. Unique Identifier : AIDSLINE
       MED/95309975
 AB    We investigated the cellular requirement for primary in vitro generation
       of cytotoxic T-lymphocytes (CTL) in BALB/c spleen cells against
       Ld-binding self-peptide p2Ca. Depletion of CD4+ T-cells in vitro by
       pretreatment with anti-CD4 monoclonal antibody (mAb) and complement or
       in vivo by administration of anti-CD4 mAb abrogated generation of CTL.
       Depletion of adherent cells by passing spleen cells through a nylon wool
       (NW) column also abrogated generation of CTL. Addition of peritoneal
       exudate cells (PEC) to spleen cells passed through the NW column
       restored CTL generation. These findings indicate that both CD4+ T-cells
       and antigen-presenting cells (APC) were necessary for CTL generation.
       Treatment of PEC with paraformaldehyde (PFA), but not mitomycin-C (MMC)
       abrogated their ability to restore CTL generation when mixed with spleen
       cells from the NW column, suggesting that an endocytic pathway could be
       involved in presentation of p2Ca on APC.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal/IMMUNOLOGY
       Antigen-Presenting Cells/*IMMUNOLOGY  Binding, Competitive  Cell
       Communication/IMMUNOLOGY  Cytotoxicity, Immunologic  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY  CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Mice
       Mice, Inbred BALB C  Mice, Inbred C57BL  Molecular Sequence Data
       Oligopeptides/*IMMUNOLOGY  Spleen/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).