Document 1178
 DOCN  M9591178
 TI    Human alpha beta T-cell receptor CD4-CD8 T-cell clones are predominantly
       Th0-like.
 DT    9509
 AU    Katsikis PD; Cohen SB; Murison JG; Uren J; Hibbart LM; Callard RE; Di
       Padova F; Feldmann M; Londei M; Mathilda & Terence Kennedy Institute of
       Rheumatology, Sunley; Division, Hammersmith, London, UK.
 SO    Immunology. 1995 Apr;84(4):501-4. Unique Identifier : AIDSLINE
       MED/95309956
 AB    The cytokine production profile, focusing on interleukin-4 (IL-4),
       interferon-gamma (IFN-gamma) and interleukin-10 (IL-10) of human CD4+,
       CD8+ and CD4- CD8- alpha beta T cells cloned from the peripheral blood
       of healthy individuals was compared. Solid-phase anti-CD3 stimulation of
       CD4- CD8- alpha beta T cell clones from one individual revealed a
       significantly increased frequency of IL-4-producing clones (81%),
       compared to CD4+ T cells (24%) or CD8+ (28%). All five CD4- CD8- alpha
       beta T-cell clones from two other individuals also produced IL-4. Clones
       that produced IFN-gamma with undetectable IL-4 production, thus being of
       the 'classical' Th1 phenotype, were infrequent in CD4- CD8- alpha beta
       T-cell clones (19%) compared to CD4+ (71%), and CD8+ clones (72%) cloned
       in OKT3, and CD4+ cells cloned in phytohaemaglutinin A (77%). Unlike
       previously reported studies with gamma delta cells, the alpha beta CD4-
       CD8- T cells produced IL-10 at appreciable frequency (38%) in PHA
       generated clones. The supernatants from anti-CD3 stimulated CD4- CD8-
       alpha beta T-cell clones contained sufficient IL-4 to activate B cells,
       enhancing CD23 and surface immunoglobulin M (IgM) expression and
       co-stimulating B-cell proliferation. These findings suggest that the
       function of CD4- CD8- alpha beta T cells is distinct from that of most
       CD4+ or CD8+ T cells.
 DE    B-Lymphocytes/IMMUNOLOGY  Cells, Cultured  Clone Cells/IMMUNOLOGY
       CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Human  Interferon Type II/*BIOSYNTHESIS
       Interleukin-10/BIOSYNTHESIS  Interleukin-4/BIOSYNTHESIS
       Interleukins/*BIOSYNTHESIS  Lymphocyte Transformation  Receptors,
       Antigen, T-Cell, alpha-beta/*ANALYSIS  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).