Document 1194
 DOCN  M9591194
 TI    The effect of interleukin-2 on suppressor T lymphocytes in autoimmune
       thyroid disease.
 DT    9509
 AU    Yoshikawa N; Morita T; Arreaza G; Resetkova E; Mukuta T; Volpe R;
       Department of Medicine, Wellesley Hospital, University of; Toronto,
       Ontario.
 SO    Clin Invest Med. 1995 Apr;18(2):91-8. Unique Identifier : AIDSLINE
       MED/95308799
 AB    We have investigated the effects of interleukin-2 (IL-2) on the
       activation of suppressor T lymphocytes in autoimmune thyroid disease
       (AITD), with insulin-dependent diabetes mellitus (IDDM) as an autoimmune
       disease control; this was accomplished by measuring the expression of
       major histocompatibility complex class II (HLA-DR), CD25 (IL-2 alpha
       receptor (R)), and IL-2 beta R expression on their surfaces by flow
       cytometric analysis. Peripheral blood mononuclear cells (PBMC), obtained
       from 10 patients with Graves' disease (GD), 11 with Hashimoto's
       thyroiditis (HT), 9 with insulin-dependent diabetes mellitus (IDDM), and
       10 normal persons (N), were cultured for 7 d in the presence or absence
       of IL-2 at a final concentration of 50 U/mL. CD8+ cells were isolated
       from cultured PBMC with immunomagnetic beads, and were stained with
       fluorescent-conjugated monoclonal antibodies (anti-CD11b, anti-IL-2
       alpha R, anti-IL-2 beta R, and anti-HLA-DR); the activation of
       CD8+CD11b+ (suppressor) T cells (Ts) by IL-2 was then analyzed on a flow
       cytometer. In the absence of IL-2, i.e., in the autologous mixed
       lymphocyte reaction (AMLR), Ts from patients with GD, HT, and IDDM
       showed significantly lower activation as compared to N when analyzed by
       HLA-DR expression, but were not significantly different when IL-2R
       expression was measured. We determined the Stimulation Index (SI) of the
       activation of T lymphocytes by IL-2 for comparison between N and
       patients. With stimulation of 50 U/mL of IL-2, SI of HLA-DR+ Ts was
       significantly (p < 0.05 to 0.01) lower in GD, HT, and IDDM as compared
       with N.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Adult  Aged  Antibodies, Monoclonal/IMMUNOLOGY  Antigens, CD11/ANALYSIS
       CD8-Positive T-Lymphocytes/IMMUNOLOGY  Diabetes Mellitus,
       Insulin-Dependent/IMMUNOLOGY  Female  Graves' Disease/*IMMUNOLOGY  Human
       HLA-DR Antigens/ANALYSIS  Interleukin-2/*PHARMACOLOGY  Lymphocyte
       Culture Test, Mixed  Lymphocyte Transformation  Male  Middle Age
       Support, Non-U.S. Gov't  T-Lymphocytes, Suppressor-Effector/*IMMUNOLOGY
       Thyroiditis, Autoimmune/*IMMUNOLOGY  Triiodothyronine/BLOOD  JOURNAL
       ARTICLE

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