Document 1220
 DOCN  M9591220
 TI    Diminished expression of cell-surface complement regulatory proteins in
       HIV-infected children and with HIV infection of peripheral blood
       mononuclear cells in vitro.
 DT    9509
 AU    Jarvis JN; Taylor H; Long PM; Gutta PV; Pousak T; Fine N; Department of
       Pediatrics/Immunology, Wayne State University; School of
       Medicine/Children's Hospital of Michigan 48201, USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jul 1;9(3):249-56.
       Unique Identifier : AIDSLINE MED/95308200
 AB    Experimental data have established that HIV-infected lymphocytes
       activate the complement system. However, because mammalian lymphocytes
       possess a series of cell-surface complement regulators that inhibit
       amplification on autologous cells, complement-mediated destruction of
       host cells is usually inhibited. These studies were performed to examine
       whether alterations in the cell-surface complement regulatory proteins
       decay-accelerating factor (DAF, CD55) and membrane cofactor protein
       (MCP, CD46) may occur during HIV infection in vitro or in vivo. The
       physiologic significance of these alterations were assessed by
       radiolabeled chromium release experiments. We show that MCP fluorescent
       intensity is significantly lessened in HIV-infected children and that
       DAF intensity is similarly lessened in infected children with advanced
       disease. These findings could be duplicated with HIV infection of
       peripheral blood mononuclear cells in vitro.
 DE    Adult  Antigens, CD/*BIOSYNTHESIS  Cell Line  Cells, Cultured  Child,
       Preschool  Complement/BIOSYNTHESIS  Complement
       Inactivators/*BIOSYNTHESIS  Enzyme-Linked Immunosorbent Assay  Female
       Fluorescent Antibody Technique  Human  HIV Infections/*IMMUNOLOGY
       *HIV-1  Infant  Leukocytes, Mononuclear/*IMMUNOLOGY/VIROLOGY  Male
       Membrane Glycoproteins/*BIOSYNTHESIS  Receptors, Virus/BIOSYNTHESIS
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

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