Document 1223
 DOCN  M9591223
 TI    Human CD4+ T cells can discriminate the molecular and structural context
       of T epitopes of HIV gp120 and HIV p66.
 DT    9509
 AU    Manca F; Fenoglio D; Valle MT; Li Pira G; Kunkl A; Ferraris A; Saverino
       D; Lancia F; Mortara L; Lozzi L; et al; Department of Immunology, San
       Martino Hospital, University of; Genoa, Italy.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jul 1;9(3):227-37.
       Unique Identifier : AIDSLINE MED/95308197
 AB    CD4+ T cell lines and clones specific for human immunodeficiency virus
       (HIV) antigens have been generated from peripheral lymphocytes of naive
       individuals by priming with the envelope protein gp120, the enzyme
       reverse transcriptase (p66), and their synthetic peptides. T cells were
       tested for proliferation to proteins, to peptides, and to HIV virions.
       Different patterns of reaction were identified. T cells primed in vitro
       with the whole antigen responded to the protein, but recognition of
       overlapping peptides occurred with a fraction of the lines or clones.
       The virus was recognized by some, but not all, of the gp120- and
       p66-specific T cells, with an efficiency 2 logs higher than the
       recombinant soluble proteins on a molar basis. One T cell line specific
       for gp120 responded to virions presented by B cells, but not by
       monocytes. In contrast, T cells induced with peptides did not always
       respond to the proteins. Generation of T cell lines from naive
       individuals may be an in vitro model for T cell immunization, and the
       response patterns may have implications for the design of vaccines aimed
       at inducing a T helper response. In fact our in vitro data suggest that
       (a) immunization with peptides does not always induce T cells
       recognizing the whole protein, (b) immunization with proteins does not
       always induce T cells recognizing the protein in the context of the HIV
       virus, and (c) recognition of gp120 in the context of HIV may be
       dictated by the type of presenting cells.
 DE    Amino Acid Sequence  Antigen-Presenting Cells/IMMUNOLOGY  Antigenic
       Determinants/ANALYSIS  B-Lymphocytes/IMMUNOLOGY  Cell Line  Clone Cells
       CD4-Positive T-Lymphocytes/*IMMUNOLOGY  Human  HIV Antigens/*IMMUNOLOGY
       HIV Envelope Protein gp120/CHEMISTRY/*IMMUNOLOGY  HIV Envelope Protein
       gp41/CHEMISTRY/IMMUNOLOGY  HIV-1/ENZYMOLOGY/*IMMUNOLOGY  Lymphocyte
       Transformation/IMMUNOLOGY  Molecular Sequence Data  Monocytes/IMMUNOLOGY
       Peptide Fragments/CHEMISTRY/IMMUNOLOGY  Reverse
       Transcriptase/CHEMISTRY/*IMMUNOLOGY  Structure-Activity Relationship
       Support, Non-U.S. Gov't  Virion/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).