Document 1224
 DOCN  M9591224
 TI    Multiple superinfections fail to activate defective human
       immunodeficiency virus-1 (HIV-1) infection of rabbits.
 DT    9509
 AU    Sell S; Tseng CK; Department of Pathology, University of Texas Houston
       77225, USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jul 1;9(3):211-26.
       Unique Identifier : AIDSLINE MED/95308196
 AB    Superinfection of human immunodeficiency virus (HIV)-1-infected rabbits
       with Treponema pallidum, Mycobacterium avium, herpes simplex, Candida
       albicans, Mycoplama incognitus, and malignant catarrhal fever virus, as
       well as irradiation or cortisone treatment, fails to activate production
       of infectious virus. For up to 6 months after infection of rabbits with
       HIV-infected cells or free virus, there are neither clinical symptoms
       nor positive laboratory tests for detection of HIV. However, after
       superinfection with other agents, HIV sequences may be transiently found
       in peripheral blood mononuclear cells by polymerase chain reaction
       (PCR), and multiple antibodies to HIV antigens may be detected by
       Western blotting. Both the PCR positivity and Western blot reactivity
       become negative with time after superinfection. Other than delayed
       healing of the skin lesions produced by T. pallidum and vaccinia in
       HIV-infected rabbits, there is no evidence of any immune abnormality.
       After death, gag sequences are detectable in the splenocytes of
       essentially every HIV-infected rabbit, and the splenocytes of eight of
       25 infected rabbits responded by proliferation to HIV peptides. In
       addition, gag sequences are detectable in rabbits that are injected with
       lymphoid cells from HIV-infected rabbits. However, after multiple
       testing of both peripheral blood of living rabbits and organs of rabbits
       that died or were killed (spleen, brain, lymph nodes, liver, and
       gastrointestinal tract), no viable virus has ever been convincingly
       detected by in vitro cultivation with indicator cells. In contrast to
       some other published reports, these data indicate that HIV-1 infection
       of rabbits does not provide a model for AIDS pathogenesis therapy or
       prevention, but it may be useful as a model to study the relative
       resistance of a small fraction of the human population to development of
       AIDS after HIV infection.
 DE    Animal  Bacterial Infections/PHYSIOPATHOLOGY
       Candidiasis/PHYSIOPATHOLOGY  Cell Line  Cortisone/PHARMACOLOGY
       Defective Viruses/*PHYSIOLOGY  DNA, Viral/ANALYSIS  Gene Products,
       gag/ANALYSIS  Human  HIV Antibodies/BLOOD  HIV Antigens/IMMUNOLOGY  HIV
       Infections/*PHYSIOPATHOLOGY  HIV-1/DRUG EFFECTS/ISOLATION &
       PURIF/*PHYSIOLOGY/RADIATION  EFFECTS  Immunoblotting  Leukocytes,
       Mononuclear/PHYSIOLOGY/VIROLOGY  Male  Polymerase Chain Reaction
       Rabbits  Superinfection/*PHYSIOPATHOLOGY  Support, Non-U.S. Gov't  Virus
       Activation/*PHYSIOLOGY  Virus Diseases/PHYSIOPATHOLOGY  Virus Latency
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).