Document 1265
 DOCN  M9591265
 TI    Evaluation of HIV type 1 western blot-indeterminate blood donors for the
       presence of human or bovine retroviruses.
 DT    9509
 AU    Sherman MP; Dock NL; Ehrlich GD; Sninsky JJ; Brothers C; Gillsdorf J;
       Bryz-Gornia V; Poiesz BJ; Department of Medicine, State University of
       New York, Syracuse; 13210, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Mar;11(3):409-14. Unique Identifier :
       AIDSLINE MED/95306145
 AB    From 1985 through 1990, 1100 of 500,000 human blood donations in
       Syracuse, New York were repeatedly reactive by ELISA for antibodies to
       the human immunodeficiency virus type 1 (HIV-1). Nine hundred of the
       ELISA-reactive samples were confirmed as negative by Western blot (WB),
       40 were confirmed as positive, and the remaining 160 sera were
       indeterminate, reacting mainly with HIV-1 gag gene products. Twenty
       donors with the most reactive indeterminate WB were selected for
       follow-up studies. Four of these 20 donors admitted to retroviral risk
       factors and, interestingly, 12 (60%) had exposure to dairy cattle and
       drank unpasteurized milk. These 20 donors were analyzed over a 3-year
       period for the presence of the pathogenic human retroviruses HIV-1,
       HIV-2, human T cell lymphoma/leukemia virus types I and II (HTLV-I and
       HTLV-II), as well as bovine immunodeficiency virus (BIV) and leukemia
       virus (BLV). Retroviral analyses included serology, plasma antigen
       capture, virus culture, and the polymerase chain reaction. Only one
       donor seroconverted and was clearly infected with HIV-1. None of the
       other 19 donor serological reactivities to HIV-1 changed, nor were they
       positive for any of the above-mentioned retroviruses. Although we cannot
       ascertain whether these latter 19 HIV-1 WB-indeterminate donors were
       exposed to human or bovine retroviral proteins, it is unlikely that
       their HIV-1 seroreactivity was caused by infection with HIV-1, HIV-2,
       HTLV-I, HTLV-II, BLV, or BIV.
 DE    Acquired Immunodeficiency Syndrome/EPIDEMIOLOGY/*PREVENTION &  CONTROL
       Animal  Base Sequence  *Blood Donors  Blotting, Western/*METHODS  Cattle
       Comparative Study  DNA Primers  Enzyme-Linked Immunosorbent
       Assay/METHODS  False Negative Reactions  False Positive Reactions
       Female  Gene Products, gag/*BLOOD  Genes, gag  Human  HIV Seronegativity
       HIV Seropositivity/*DIAGNOSIS  HIV-1/GENETICS/*ISOLATION & PURIF
       HIV-2/ISOLATION & PURIF  HTLV-I/ISOLATION & PURIF  HTLV-II/ISOLATION &
       PURIF  Immunodeficiency Virus, Bovine/ISOLATION & PURIF  Milk/*VIROLOGY
       Molecular Sequence Data  New York  Polymerase Chain Reaction/METHODS
       Reproducibility of Results  Retroviridae
       Infections/*DIAGNOSIS/EPIDEMIOLOGY  Risk Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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