Document 1266
 DOCN  M9591266
 TI    Polyclonal rabbit antisera that detect the Vpr protein of SIVSM and
       SIVMAC on immunoblots of purified virions.
 DT    9509
 AU    Newman MA; McPherson SA; Fletcher TM 3rd; Kappes JC; Hahn BH; Department
       of Medicine, University of Alabama at Birmingham; 35294, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Mar;11(3):405-8. Unique Identifier :
       AIDSLINE MED/95306144
 AB    Antisera suitable for detection of SIVSM or SIVMAC Vpr proteins on
       Western blots of purified virions are currently not available. We have
       expressed the Vpr protein of SIVSMPBj1.9 in a gst-based prokaryotic
       expression system and used it to raise polyclonal antisera in rabbits.
       Two immune sera were obtained that specifically recognized both cell-
       and virion-associated Vpr protein on immunoblots of three different SIV
       isolates (SIVSMPBj1.9, SIVMACBK28, and SIVMAC239). Because Vpr is
       believed to play an important role in HIV/SIV replication and
       pathogenesis, these reagents will allow the extension of functional
       analyses of this protein to a broader spectrum of viruses. Both antisera
       and the gst-Vpr expression plasmid have been submitted to the NIAID AIDS
       Research and Reagent Program and are available to interested
       investigators.
 DE    Amino Acid Sequence  Animal  *Antibodies  Base Sequence  Cell Line
       Cercopithecus aethiops  DNA Primers  Electrophoresis, Polyacrylamide Gel
       Gene Products, vpr/*ANALYSIS/*BIOSYNTHESIS  Human  Immunoblotting
       Molecular Sequence Data  Polymerase Chain Reaction  Rabbits/IMMUNOLOGY
       Recombinant Proteins/ANALYSIS/BIOSYNTHESIS  Support, Non-U.S. Gov't
       Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.
       SIV/CHEMISTRY/GENETICS/*PHYSIOLOGY  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).