Document 1267
 DOCN  M9591267
 TI    Analysis of envelope glycoprotein-specific antibodies from SIV-infected
       and gp110-immunized monkeys in ACC and ADCC assays.
 DT    9509
 AU    Ohkawa S; Xu K; Wilson LA; Montelaro R; Martin LN; Murphey-Corb M;
       Department of Microbiology, Tulane Regional Primate Research; Center,
       Covington, Louisiana 70433, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Mar;11(3):395-403. Unique Identifier :
       AIDSLINE MED/95306143
 AB    Sera collected from SIV-infected or recombinant glycoprotein-immunized
       monkeys were characterized for antibodies participating in
       antibody-complement-mediated cytolysis (ACC) and antibody-dependent
       cellular cytolysis (ADCC) in terms of their IgG subclass and epitope
       specificity. In a competitive inhibition ELISA, gp110-specific antibody
       reactivity with nondenatured rgp110 was blocked completely by soluble
       homologous rgp110 and partially inhibited by heterologous rgp110,
       suggesting cross-reactivity between viral strains. However, only partial
       inhibition was observed with denatured recombinant gp140 (rgp140) in
       selected monkeys, indicating that the majority of gp110-specific
       antibodies recognized conformational epitopes. ACC activity against
       recombinant vaccinia-infected, envelope-expressing targets was found in
       sera from both infected and immunized monkeys, whereas ADCC activity was
       observed only in sera from infected monkeys. ACC was blocked with
       denatured rgp140 as well as nondenatured rgp110, indicating that
       ACC-mediating antibodies recognized mainly linear epitopes. In contrast,
       rgp140 did not compete as effectively as rgp110 in the ADCC assay,
       indicating that the majority of ADCC antibodies recognized
       conformational epitopes. Competitive inhibition using three peptide
       fragments of gp110 indicated that epitopes recognized by ACC antibodies
       lie within amino acid residues 214-471, a region that spans V3, whereas
       ADCC-reactive epitopes lie between amino acid residues 52 and 214 at the
       N-terminal end of gp110. Column chromatography of rhesus IgG resulted in
       three subclass-enriched fractions, designated IgG-I, IgG-II, and
       IgG-III. IgG-I, but not IgG-II or IgG-III, from both infected and
       immunized monkeys mediated ACC, whereas IgG-I and IgG-II from infected
       monkeys mediated ADCC.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Animal  Antibodies, Viral/*BLOOD  *Antibody-Dependent Cell Cytotoxicity
       Antigen-Antibody Reactions  Binding, Competitive  Cell Line  Cercocebus
       Cercopithecus aethiops  Comparative Study  *Cytotoxicity, Immunologic
       Enzyme-Linked Immunosorbent Assay  IgG/*BLOOD/CLASSIFICATION
       Immunization  Macaca mulatta  Recombinant Proteins/IMMUNOLOGY  Simian
       Acquired Immunodeficiency Syndrome/BLOOD/*IMMUNOLOGY  Support, U.S.
       Gov't, P.H.S.  SIV/*IMMUNOLOGY  Viral Envelope Proteins/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).