Document 1273
 DOCN  M9591273
 TI    Increase in sensitivity to soluble CD4 by primary HIV type 1 isolates
       after passage through C8166 cells: association with sequence differences
       in the first constant (C1) region of glycoprotein 120.
 DT    9509
 AU    Orloff SL; Bandea CI; Kennedy MS; Allaway GP; Maddon PJ; McDougal JS;
       Immunology Branch, Public Health Service, U.S. Department of; Health and
       Human Services, Atlanta, Georgia 30333, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Mar;11(3):335-42. Unique Identifier :
       AIDSLINE MED/95306137
 AB    Primary isolates of human immunodeficiency virus type 1 (HIV-1) were
       obtained by coculture of peripheral blood lymphocytes (PBLs) from
       HIV-1-infected people with PBLs from uninfected donors. These viral
       stocks tend to be resistant to neutralization/inactivation by soluble
       CD4 (sCD4). When these stocks were passed through the T cell line C8166,
       virus stocks emerged that were sensitive to sCD4. Pre- and post-C8166
       stocks maintained their sCD4-resistant and -sensitive phenotypes,
       respectively, with further passage in PBLs. Pre- and post-C8166 stocks
       were biologically cloned by two cycles of limiting dilution. The
       majority (14 of 17) of pre-C8166 clones were sCD4 resistant, and,
       conversely, the majority of post-C8166 clones (11 of 12) were sensitive
       to sCD4. Nucleotide and predicted amino acid sequence analysis in the
       env (gp120) region revealed a limited number of differences between the
       clones. The only differences that sorted with biological phenotype were
       in the first constant (C1) region of gp120. Adaptation to growth in
       C8166 cells and conversion from the sCD4-resistant to the sCD4-sensitive
       phenotype represent the emergence to prominence of viral species in the
       pre-C8166 stock that have a replication advantage in C8166 coincident
       with increased sensitivity to sCD4.
 DE    Amino Acid Sequence  Antigens, CD/*PHYSIOLOGY  Antigens, CD4/*PHYSIOLOGY
       Cell Line  Cells, Cultured  DNA, Viral/METABOLISM  Giant Cells  Human
       HIV Seropositivity/*VIROLOGY  HIV-1/ISOLATION &
       PURIF/*PHYSIOLOGY/PATHOGENICITY  Kinetics
       Lymphocytes/*IMMUNOLOGY/*VIROLOGY  Male  Molecular Sequence Data
       Phenotype  Polymerase Chain Reaction
       Proviruses/GENETICS/PHYSIOLOGY/PATHOGENICITY  Virus Activation  JOURNAL
       ARTICLE

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