Document 1274
 DOCN  M9591274
 TI    Human immunodeficiency virus type 1-neutralizing antibodies raised to a
       glycoprotein 41 peptide expressed on the surface of a plant virus.
 DT    9509
 AU    McLain L; Porta C; Lomonossoff GP; Durrani Z; Dimmock NJ; Department of
       Biological Sciences, University of Warwick,; Coventry, UK.
 SO    AIDS Res Hum Retroviruses. 1995 Mar;11(3):327-34. Unique Identifier :
       AIDSLINE MED/95306136
 AB    An oligonucleotide encoding the amino acids 731-752 of the gp41 envelope
       protein of the human immunodeficiency virus type 1 strain IIIB, which is
       known to induce cross-reactive neutralizing antibodies in humans, was
       inserted into a full-length clone of the RNA encoding the coat proteins
       of cowpea mosaic virus (RNA 2 of CPMV). When transfected together with
       RNA 1 of CPMV, transcribed RNA 2 was able to replicate in plants and
       form infectious virions (CPMV-HIV). Purified virions were injected
       subcutaneously with alum adjuvant into adult C57/BL6 mice to determine
       their ability to stimulate ELISA and neutralizing antibody specific for
       HIV-1. Antisera to CPMV-HIV obtained after only two injections gave a
       strong ELISA response (mean of 1:25,800) using the free gp41 peptide as
       antigen, showing that the gp41 peptide incorporated into the chimera was
       immunogenic. The same antisera gave 97% neutralization of HIV-1 IIIB at
       1:100 dilution, with a highly uniform response in all (six of six)
       animals tested. A third injection barely increased the neutralization
       titer. Normal mouse serum had no neutralizing activity. Antisera also
       strongly neutralized the HIV-1 strains RF and SF2. ELISA and
       neutralizing activity to HIV-1 IIIB declined after the second injection
       and were undetectable after 7 weeks, but were restimulated to the same
       level after the third injection. Neutralization was marginally more
       stable after the third injection. Antibody specific for CPMV epitopes
       was equally short lived. A bonus of this system was unexpected
       neutralizing activity specifically stimulated by unmodified CPMV
       virions, although this amounted to no more than 10% of the neutralizing
       activity stimulated by the CPMV-HIV chimera.(ABSTRACT TRUNCATED AT 250
       WORDS)
 DE    Animal  Antibody Specificity  Capsid/BIOSYNTHESIS  Cell Line  Cloning,
       Molecular  Comovirus/*GENETICS/PHYSIOLOGY/ULTRASTRUCTURE  Cross
       Reactions  Enzyme-Linked Immunosorbent Assay  Human  HIV
       Antibodies/*IMMUNOLOGY  HIV Antigens/BIOSYNTHESIS/*IMMUNOLOGY  HIV
       Envelope Protein gp41/BIOSYNTHESIS/*IMMUNOLOGY
       HIV-1/GENETICS/*IMMUNOLOGY/ULTRASTRUCTURE  Mice  Mice, Inbred C57BL
       Neutralization Tests  Recombinant Proteins/BIOSYNTHESIS/IMMUNOLOGY
       Recombination, Genetic  Support, Non-U.S. Gov't
       Virion/GENETICS/PHYSIOLOGY/ULTRASTRUCTURE  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).