Document 1278
 DOCN  M9591278
 TI    An approach for an immunoaffinity AIDS sensor using the conservative
       region of the HIV envelope protein (gp41) and its monoclonal antibody.
 DT    9509
 AU    Uda T; Hifumi E; Kobayashi T; Shimizu K; Sata T; Ogino K; Hiroshima
       Prefectural University, School of Bioscience, Shoubara; city, Japan.
 SO    Biosens Bioelectron. 1995;10(5):477-83. Unique Identifier : AIDSLINE
       MED/95306021
 AB    A monoclonal antibody for the conservative region of gp41, which is one
       of the HIV envelope proteins, was produced. The antigen determining site
       of gp41 was examined using the epitope mapping technique, followed by an
       enzyme linked immunosorbent assay. Some peptides had comparable
       affinities for the monoclonal antibody, but the peptide EGIEE, having a
       slightly weaker immunoaffinity than gp41, was the most preferable for
       the construction of an immunoaffinity AIDS sensor. For the detection of
       gp41, EGIEE was labelled with catalase and used as a mimic antigen; it
       was bound to the antibody present on an immuno-membrane and, due to the
       replacement reaction of the mimic antigen by gp41, indirect quantitative
       measurement of gp41 was possible using an oxygen electrode. Anti-gp41
       antibody was also detected using a mimic antibody, which was chemically
       modified with polyethylene glycol. An immunoaffinity AIDS sensor was
       constructed using the mimic molecules which were tailored to have a
       suitable immunoaffinity for HIV antigen and/or antibody.
 DE    Acquired Immunodeficiency Syndrome/*DIAGNOSIS  Amino Acid Sequence
       Antibodies, Monoclonal/*IMMUNOLOGY  *Biosensors  Epitope Mapping  Human
       HIV Envelope Protein gp41/*ANALYSIS/IMMUNOLOGY  Molecular Sequence Data
       Protein Conformation  JOURNAL ARTICLE

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