Document 0019
 DOCN  M95A0019
 TI    Transactivation of human T-cell leukemia virus type 1 by herpes simplex
       virus type 1.
 DT    9510
 AU    Israel S; Mendelovitz M; Honigman A; Department of Molecular Genetics,
       Hebrew University-Hadassah; Medical School, Jerusalem, Israel.
 SO    Virus Genes. 1995 Feb;9(3):269-76. Unique Identifier : AIDSLINE
       MED/95320972
 AB    HTLV-1 transcription depends upon activation by the HTLV-1 tax gene
       product. In addition, various substances and cellular transcription
       factors are also known to activate the HTLV-1 long terminal repeat
       (LTR)-mediated transcription in the absence of Tax. In this work we
       demonstrate that infection of either Jurkat or 293 cell lines with
       herpes simplex I (HSV-1), a widespread infectious virus of humans,
       activates HTLV-1 LTR-mediated gene expression. Further investigation
       revealed that each of the immediate-early (IE) gene products--ICPO,
       ICP4, and ICP27--of HSV-1 transactivates the HTLV-1 LTR-mediated gene
       expression in the absence of Tax. The HSV-1 activation is additive to
       Tax activation in its presence in the cell. Three 21 base repeats
       upstream of the TATA box are known as the TAX responsive elements (TRE).
       Recombinant HTLV-1 minimal promoter composed of the HTLV-1 TATA box
       fused to a synthetic 21 base TRE is responsive to Tax but not to HSV-1
       activation. It thus can be concluded that HSV-1 IE gene products and Tax
       transactivates HTLV-1 LTR mediated gene expression through different
       transcription complexes. The results presented in this work may point to
       one possible way for the transition of HTLV-1 from a quiescent to an
       actively replicating stage.
 DE    Base Sequence  *Gene Expression Regulation, Viral  Gene Products,
       tax/PHYSIOLOGY  Genes, Immediate-Early  Genes, Viral  Human
       HTLV-I/*GENETICS  Immediate-Early Proteins/PHYSIOLOGY  Leukemia, T-Cell,
       Acute/PATHOLOGY  Molecular Sequence Data  Repetitive Sequences, Nucleic
       Acid  Simplexvirus/*GENETICS  Species Specificity  Support, Non-U.S.
       Gov't  *Trans-Activation (Genetics)  Tumor Cells, Cultured  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).