Document 0044
 DOCN  M95A0044
 TI    Inflammatory mediators increase surface expression of integrin ligands,
       adhesion to lymphocytes, and secretion of interleukin 6 in mouse Sertoli
       cells.
 DT    9510
 AU    Riccioli A; Filippini A; De Cesaris P; Barbacci E; Stefanini M; Starace
       G; Ziparo E; Institute of Histology and General Embryology, School of
       Medicine; La Sapienza University, Rome, Italy.
 SO    Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):5808-12. Unique Identifier
       : AIDSLINE MED/95320164
 AB    The expression of the cell adhesion molecules ICAM-1, ICAM-2, and VCAM-1
       and the secretion of the cytokine interleukin 6 have been measured in
       mouse Sertoli cells cultured in vitro. Cytometric analysis revealed
       that, in basal conditions, low levels of ICAM-1 and VCAM-1 were present
       on the surface of the cells, whereas treatment with interleukin 1, tumor
       necrosis factor alpha, lipopolysaccharide, or interferon gamma induced,
       with different kinetics, increases in their expression. ICAM-2 was not
       detectable in basal conditions, nor was it inducible. Electron
       microscopic analysis and binding experiments using 51Cr-labeled
       lymphocytes demonstrated that increased expression of ICAM-1 and VCAM-1
       on the surface of Sertoli cells, induced by inflammatory mediators,
       determines an augmented adhesion between the two cell types. The same
       stimuli, with the exception of interferon gamma, produced a rapid and
       remarkable increment of interleukin 6 production by Sertoli cells. These
       results suggest the presence of both direct and paracrine mechanisms of
       interaction between Sertoli and immune-competent cells, possibly
       involved in the control of immune reactions in the testis. Such
       mechanisms are of interest for the understanding of autoimmune
       pathologies of the testis and, if confirmed in humans, they could be
       involved in the sexual transmission of human immunodeficiency virus
       infection.
 DE    Animal  Antigens, CD/BIOSYNTHESIS  *Cell Adhesion  Cell Adhesion
       Molecules/*BIOSYNTHESIS  Cell Membrane/IMMUNOLOGY/ULTRASTRUCTURE  Cells,
       Cultured  Cytokines/*PHARMACOLOGY  *Gene Expression/DRUG EFFECTS
       Inflammation  Intercellular Adhesion Molecule-1/BIOSYNTHESIS
       Interferon-gamma, Recombinant/PHARMACOLOGY  Interleukin-1/PHARMACOLOGY
       Interleukin-6/*BIOSYNTHESIS  Lipopolysaccharides/PHARMACOLOGY
       Lymphocytes/IMMUNOLOGY/ULTRASTRUCTURE  Male  Mice  Mice, Inbred Strains
       Microscopy, Electron, Scanning  Sertoli Cells/DRUG
       EFFECTS/*IMMUNOLOGY/ULTRASTRUCTURE  Spleen/IMMUNOLOGY  Support, Non-U.S.
       Gov't  Tumor Necrosis Factor/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).