Document 0046
 DOCN  M95A0046
 TI    Persistent high frequency of human immunodeficiency virus-specific
       cytotoxic T cells in peripheral blood of infected donors.
 DT    9510
 AU    Moss PA; Rowland-Jones SL; Frodsham PM; McAdam S; Giangrande P;
       McMichael AJ; Bell JI; Institute of Molecular Medicine, John Radcliffe
       Hospital,; Headington, Oxford, United Kingdom.
 SO    Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):5773-7. Unique Identifier :
       AIDSLINE GENBANK/U26673
 AB    Human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes
       (CTLs) are thought to play a major role in the immune response to HIV
       infection. The HIV-specific CTL response is much stronger than
       previously documented in an infectious disease, yet estimates of CTL
       frequency derived from limiting-dilution analysis (LDA) are relatively
       low and comparable to other viral infections. Here we show that
       individual CTL clones specific for peptides from HIV gag and pol gene
       products are present at high levels in the peripheral blood of three
       infected patients and that individual CTL clones may represent between
       0.2% and 1% of T cells. Previous LDA in one donor had shown a frequency
       of CTL precursors of 1/8000, suggesting that LDA may underestimate CTL
       effector frequency. In some donors individual CTL clones persisted in
       vivo for at least 5 years. In contrast, in one patient there was a
       switch in CTL usage suggesting that different populations of CTLs can be
       recruited during infection. These data imply strong stimulation of CTLs,
       potentially leading some clones to exhaustion.
 DE    Amino Acid Sequence  Base Sequence  Clone Cells  DNA Primers  Gene
       Products, gag/BIOSYNTHESIS/GENETICS  Gene Products,
       pol/BIOSYNTHESIS/GENETICS  Genes, gag  Genes, pol
       Hemophilia/COMPLICATIONS  Human  HIV/GENETICS/*IMMUNOLOGY/ISOLATION &
       PURIF  HIV Infections/*IMMUNOLOGY/TRANSMISSION/VIROLOGY  Molecular
       Sequence Data  Polymerase Chain Reaction  Receptor-CD3 Complex, Antigen,
       T-Cell/BIOSYNTHESIS/GENETICS  Receptors, Antigen, T-Cell,
       alpha-beta/BIOSYNTHESIS/GENETICS  Support, Non-U.S. Gov't
       T-Lymphocytes, Cytotoxic/*IMMUNOLOGY/*VIROLOGY  Transcription, Genetic
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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