Document 0101
 DOCN  M95A0101
 TI    Ex vivo expansion of CD4 lymphocytes from human immunodeficiency virus
       type 1-infected persons in the presence of combination antiretroviral
       agents.
 DT    9510
 AU    Wilson CC; Wong JT; Girard DD; Merrill DP; Dynan M; An DD; Kalams SA;
       Johnson RP; Hirsch MS; D'Aquila RT; et al; Infectious Disease and
       Immunopathology Units, Massachusetts; General Hospital, Boston 02114,
       USA.
 SO    J Infect Dis. 1995 Jul;172(1):88-96. Unique Identifier : AIDSLINE
       MED/95318563
 AB    Expansion of CD4 lymphocytes from human immunodeficiency virus type 1
       (HIV-1)-infected persons ex vivo has been limited by enhanced virus
       replication and cell death. The successful expansion of functional CD4
       lymphocytes from HIV-1-infected persons has now been accomplished using
       a bispecific monoclonal antibody to CD3 and CD8 in combination with
       three antiretroviral agents. CD4 lymphocytes were polyclonally expanded
       by a factor of 10(3)-10(7) during 4-8 weeks in culture. Supernatants
       from most cultures were persistently HIV-1 p24 antigen-negative by day
       14 and remained negative despite removal of antiretroviral agents at day
       28. In such cultures, HIV-1 could not be recovered by cocultivation, and
       amounts of HIV-1 DNA declined or remained stable at low levels,
       eventually becoming undetectable in 2 cases. This approach establishes
       the feasibility of CD4 lymphocyte expansion in persons with HIV disease
       and may be useful for immune-based or gene therapies.
 DE    Acquired Immunodeficiency Syndrome/DRUG THERAPY/*IMMUNOLOGY/  VIROLOGY
       Antiviral Agents/*THERAPEUTIC USE  Base Sequence  Cells, Cultured
       Comparative Study  *CD4 Lymphocyte Count  CD4-Positive
       T-Lymphocytes/DRUG EFFECTS/*IMMUNOLOGY  Drug Therapy, Combination  DNA
       Primers  DNA, Viral/*BLOOD  Human  HIV Infections/DRUG
       THERAPY/*IMMUNOLOGY/VIROLOGY  HIV Long Terminal Repeat  HIV
       Seropositivity/DRUG THERAPY/*IMMUNOLOGY/VIROLOGY
       *HIV-1/GENETICS/ISOLATION & PURIF  Lymphocyte Transformation  Molecular
       Sequence Data  Polymerase Chain Reaction/METHODS  Receptors, Antigen,
       T-Cell, alpha-beta/ANALYSIS  Reverse Transcriptase/GENETICS  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).