Document 0112
 DOCN  M95A0112
 TI    Establishment of novel lymphoid cell lines dually infected with human T
       cell lymphotropic viruses types I and II.
 DT    9510
 AU    Kubota T; Morishita N; Tanaka Y; Sawada T; Miyagi T; Ohtsuki Y; Miyoshi
       I; Department of Medicine, Kochi Medical School, Japan.
 SO    J Infect Dis. 1995 Jul;172(1):220-4. Unique Identifier : AIDSLINE
       MED/95318527
 AB    With the goal of establishing an in vitro system of dual infection with
       human T cell lymphotropic viruses (HTLV) types I and II, rabbit
       lymphocytes were cocultured with a mixture of lethally irradiated
       HTLV-I-producing Ra-1 and HTLV-II-producing RII cell lines. This gave
       rise to a lymphoid cell line, RW-1, that was dually infected with HTLV-I
       and -II as detected by immunofluorescence staining, electron microscopy,
       and polymerase chain reaction using primers specific for the pol and env
       regions of each virus and by Southern blot hybridization. Two clonal
       cell lines derived from RW-1 were also coinfected with the viruses,
       indicating that dual infection had occurred at the single cell level.
       The coinfection could be readily propagated to fresh lymphocytes by
       coculture with RW-1. In contrast, attempts to superinfect
       HTLV-I-infected lymphoid cell lines with HTLV-II and vice versa were
       consistently unsuccessful, suggesting receptor interference between
       HTLV-I and -II.
 DE    Animal  Antigens, Surface/ANALYSIS  Antigens, Viral/ANALYSIS  Cell Line
       Cells, Cultured  DNA Primers  Female  Gene Products,
       env/ANALYSIS/BIOSYNTHESIS  Gene Products, pol/ANALYSIS/BIOSYNTHESIS
       Genes, gag  Genes, pol  Human
       HTLV-I/GENETICS/*IMMUNOLOGY/ULTRASTRUCTURE  HTLV-I
       Infections/*IMMUNOLOGY  HTLV-II/GENETICS/*IMMUNOLOGY/ULTRASTRUCTURE
       HTLV-II Infections/*IMMUNOLOGY  Lymphocytes/*IMMUNOLOGY  Microscopy,
       Electron  Polymerase Chain Reaction/METHODS  Rabbits  Support, Non-U.S.
       Gov't  Tissue Culture/METHODS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).