Document 0113
 DOCN  M95A0113
 TI    Crude extract and recombinant protein of Cryptosporidium parvum oocysts
       induce proliferation of human peripheral blood mononuclear cells in
       vitro.
 DT    9510
 AU    Gomez Morales MA; Ausiello CM; Urbani F; Pozio E; Laboratory of
       Parasitology, Istituto Superiore di Sanita, Rome,; Italy.
 SO    J Infect Dis. 1995 Jul;172(1):211-6. Unique Identifier : AIDSLINE
       MED/95318525
 AB    Cryptosporidium parvum preparations were studied for their ability to
       induce specific proliferation of cultured human peripheral blood
       mononuclear cells (PBMC) from both immunocompetent and human
       immunodeficiency virus-positive persons, some of whom had transient
       cryptosporidiosis. The proliferation of PBMC from sensitized donors
       induced by C. parvum preparations was due mainly to antigen-specific
       rather than nonspecific activation, as indicated by the kinetics of the
       proliferative response, inhibition of the PBMC proliferation by a
       monoclonal antibody directed against major histocompatibility complex
       class II-specific HLA-DR molecule, and lack of proliferation of
       umbilical cord blood PBMC. PBMC from immunocompromised patients did not
       proliferate in response to C. parvum-specific antigens. The supernatants
       of PBMC obtained from immunocompetent donors contained interleukin-10
       and interferon (IFN)-gamma after PBMC were exposed to C. parvum
       preparations. High IFN-gamma values were found in patients who had
       recovered from cryptosporidiosis, suggesting that IFN-gamma plays a role
       in resolving this infection.
 DE    Animal  Cell Division/DRUG EFFECTS  Cells, Cultured  Comparative Study
       Cryptosporidium parvum/*PATHOGENICITY  Cytokines/*BIOSYNTHESIS
       Enzyme-Linked Immunosorbent Assay  Human  Interferon Type
       II/BIOSYNTHESIS  Interleukin-10/BIOSYNTHESIS  Interleukin-2/PHARMACOLOGY
       Leukocytes, Mononuclear/*CYTOLOGY/DRUG EFFECTS/PARASITOLOGY
       Phytohemagglutinins/PHARMACOLOGY  Protozoan Proteins/*PHARMACOLOGY
       Recombinant Proteins/PHARMACOLOGY  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).