Document 0123
 DOCN  M95A0123
 TI    Molecular cloning of a new interferon-induced factor that represses
       human immunodeficiency virus type 1 long terminal repeat expression.
 DT    9510
 AU    Tissot C; Mechti N; Institut de Genetique Moleculaire de Montpellier-UMR
       9942, CNRS,; France.
 SO    J Biol Chem. 1995 Jun 23;270(25):14891-8. Unique Identifier : AIDSLINE
       GENBANK/X82200
 AB    Transcriptional induction of genes is an essential part of the cellular
       response to interferons. To isolate yet unidentified IFN-regulated genes
       we have performed a differential screening on a cDNA library prepared
       from human lymphoblastoid Daudi cells treated for 16 h with human
       alpha/beta interferon (Hu-alpha/beta IFN). In the course of these
       studies we have isolated a human cDNA which codes for a protein sharing
       homology with the mouse Rpt-1 gene; it will be referred as Staf-50 for
       Stimulated Trans-Acting Factor of 50 kDa. Amino acid sequence analysis
       revealed that Staf-50 is a member of the Ring finger family and contains
       all the features of a transcriptional regulator able to initiate a
       second cascade of gene induction (secondary response). Staf-50 is
       induced by both type I and type II IFN in various cell lines and
       down-regulates the transcription directed by the long terminal repeat
       promoter region of human immunodeficiency virus type 1 in transfected
       cells. These data are consistent with a role of Staf-50 in the mechanism
       of transduction of the IFN antiviral action.
 DE    Amino Acid Sequence  Base Sequence  Cell Line  Cloning, Molecular
       Comparative Study  DNA, Complementary  *Gene Expression Regulation,
       Viral  Hela Cells  Human  *HIV Long Terminal Repeat  HIV-1/*GENETICS
       Interferon-alpha/*PHARMACOLOGY  Interferon-beta/*PHARMACOLOGY  Molecular
       Sequence Data  Repressor Proteins/BIOSYNTHESIS/CHEMISTRY/*METABOLISM
       Sequence Homology, Amino Acid  Support, Non-U.S. Gov't  Transcription
       Factors/BIOSYNTHESIS/CHEMISTRY/*METABOLISM  Transcription, Genetic
       Transfection  Tumor Cells, Cultured  Zinc Fingers  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).