Document 0131
 DOCN  M95A0131
 TI    Productive in vitro infection of human umbilical vein endothelial cells
       and three colon carcinoma cell lines with HIV-1.
 DT    9510
 AU    Corbeil J; Evans LA; McQueen PW; Vasak E; Edward PD; Richman DD; Penny
       R; Cooper DA; Department of Pathology, University of California San
       Diego; 92093-0679, USA.
 SO    Immunol Cell Biol. 1995 Apr;73(2):140-5. Unique Identifier : AIDSLINE
       MED/95317783
 AB    The objective of this study was to assess the ability of HIV-1 to
       establish an in vitro infection of primary human umbilical vein
       endothelial cells (HUVEC). The HUVEC and colon carcinoma cell lines were
       inoculated with different isolates of HIV-1 (HIV-1SF2, HIV-1Mck and
       HIV-1LAI) and productive viral infection was assessed by both the
       detection of p24 core antigen in the culture supernatants and the
       presence of specific spliced HIV mRNA. The infection which was detected
       in the inoculated HUVEC and all the colon carcinoma cell lines could not
       be blocked using an antibody targeted against the CD4 receptor.
       Furthermore, the HIV-inoculated HUVEC secreted elevated levels of IL-6
       and this increase was found to be proportional to the size of the viral
       inoculum. No changes in the production of IL-1 beta, TNF-alpha,
       IFN-alpha and IFN-gamma were detected following HIV infection. The colon
       carcinoma cells, however, did not secrete increased levels of these
       cytokines following HIV-1 inoculation. These results confirm that
       non-CD4 expressing cells, such as endothelial cells and certain colon
       epithelial cells, serve as targets and reservoirs for HIV. Moreover, the
       production of IL-6 by HIV-infected endothelial cells may be a
       contributing factor to the aberrant immunoregulation associated with HIV
       infection in vivo.
 DE    Antigens, CD4/IMMUNOLOGY  Base Sequence  Cells, Cultured  Colonic
       Neoplasms/*VIROLOGY  Endothelium, Vascular/*VIROLOGY  Human  HIV Core
       Protein p24/BIOSYNTHESIS  HIV Infections/IMMUNOLOGY/*VIROLOGY
       HIV-1/GENETICS/IMMUNOLOGY/*PATHOGENICITY  Molecular Sequence Data  RNA,
       Viral/BIOSYNTHESIS  Support, Non-U.S. Gov't  Tumor Cells, Cultured
       Umbilical Veins/CYTOLOGY/*VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).