Document 0134
 DOCN  M95A0134
 TI    The relationship between HTLV-I-infected cell lines and uveitis.
 DT    9510
 AU    Fukushima A; Ueno H; Department of Ophthalmology, Kochi Medical School,
       Nankoku,; Japan.
 SO    Graefes Arch Clin Exp Ophthalmol. 1995 Apr;233(4):231-5. Unique
       Identifier : AIDSLINE MED/95317619
 AB    BACKGROUND: Recently it has been revealed that human T-lymphotropic
       virus type I (HTLV-I) infection causes uveitis in human. We previously
       reported HTLV-I uveitis in a rabbit. To investigate the relationship
       between HTLV-I infection and uveitis, we established an HTLV-I-infected
       T-cell clone from the cells infiltrated in the anterior chamber of this
       rabbit and compared the viral production with that in other
       HTLV-I-infected cell lines. METHODS: The clonality was determined by
       Southern blot hybridization with various restriction enzymes.
       Flow-cytometric analysis was used for investigating the expression of
       cell surface antigens. To compare viral production, we performed reverse
       transcriptase assay of the culture media and inhibition enzyme-linked
       immunosorbent assay to determine the quantity of intracellular HTLV-I
       antigens. RESULTS: The established clone was Ia (MHC class II) positive
       T cell. This T-cell clone was able to produce about three times more
       HTLV-I antigens than other HTLV-I-infected cell lines tested.
       CONCLUSION: A T-cell clone established from anterior aqueous of an
       HTLV-I uveitis rabbit can produce more HTLV-1 antigen than other
       HTLV-I-infected cell lines tested and it can be recognized easily by the
       immune system. Therefore, this high virus production may have a causal
       relation to uveitis.
 DE    Animal  Aqueous Humor/CYTOLOGY  Blotting, Southern  Cell Line  Clone
       Cells  DNA, Viral/ANALYSIS  Enzyme-Linked Immunosorbent Assay  Flow
       Cytometry  Histocompatibility Antigens Class II/ANALYSIS  Human
       HTLV-I/GENETICS/IMMUNOLOGY/*PHYSIOLOGY  HTLV-I Antigens/ANALYSIS  HTLV-I
       Infections/*COMPLICATIONS/PATHOLOGY  Rabbits  Reverse
       Transcriptase/ANALYSIS  T-Lymphocytes/IMMUNOLOGY/*VIROLOGY
       Uveitis/*ETIOLOGY/PATHOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).