Document 0223
 DOCN  M95A0223
 TI    Docosahexaenoic acid-induced alteration of Thy-1 and CD8 expression on
       murine splenocytes.
 DT    9510
 AU    Jenski LJ; Bowker GM; Johnson MA; Ehringer WD; Fetterhoff T; Stillwell
       W; Department of Biology, Indiana University-Purdue University at;
       Indianapolis, IN 46202-5132, USA.
 SO    Biochim Biophys Acta. 1995 May 24;1236(1):39-50. Unique Identifier :
       AIDSLINE MED/95315244
 AB    Here we test whether the incorporation of docosahexaenoic acid (DHA,
       22:6), an (n-3) fatty acid, into lymphocyte membranes affects the
       expression of the surface proteins Thy-1.2 and CD8. DHA was incorporated
       into splenocytes by three methods: feeding mice diets containing
       menhaden (fish) oil, fusing splenocytes with DHA-containing
       phosphatidylcholine vesicles, and culturing splenocytes with DHA.
       Thy-1.2 and CD8 expression were measured by flow cytometry and
       complement-mediated lysis using a panel of monoclonal antibodies. As
       (n-3) fatty acid incorporation into the lymphocytes increased, the
       expression of one Thy-1.2 epitope and one CD8 epitope decreased; the
       expression of two CD8 epitopes increased. Although diet-induced changes
       in surface protein expression may result from selective migration of
       cell populations or the diet's effect on protein biosynthesis, fusion
       with lipid vesicles demonstrated that DHA-containing phospholipids can
       mediate a direct and immediate effect. The decrease in Thy-1.2
       expression was sustained for more than a week after removal of (n-3)
       fatty acids from the diet, most likely due to retention of
       membrane-bound (n-3) fatty acids. Because Thy-1.2 and CD8 participate in
       T cell activation, modulation of their expression by DHA suggests that
       DHA, when serving as a membrane structural element, may alter immune
       function.
 DE    Animal  Antigens, CD8/*BIOSYNTHESIS  Antigens, Thy-1/*BIOSYNTHESIS
       Cells, Cultured  CD8-Positive T-Lymphocytes/METABOLISM  Dietary
       Fats/ADMINISTRATION & DOSAGE  Docosahexaenoic Acids/ADMINISTRATION &
       DOSAGE/*PHARMACOLOGY  Liposomes  Lymphocyte Transformation  Male  Mice
       Mice, Inbred BALB C  Spleen/DRUG EFFECTS/*METABOLISM  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).