Document 0256
 DOCN  M95A0256
 TI    HIV-1 DNA in fibroblast cultures infected with urine from
       HIV-seropositive cytomegalovirus (CMV) excretors.
 DT    9510
 AU    Margalith M; D'Aquila RT; Manion DJ; Basgoz N; Bechtel LJ; Smith BR;
       Kaplan JC; Hirsch MS; Infectious Disease Unit, Massachusetts General
       Hospital, Harvard; Medical School, Boston, USA.
 SO    Arch Virol. 1995;140(5):927-35. Unique Identifier : AIDSLINE
       MED/95328952
 AB    Interactions between HIV-1 and CMV may be important in the pathogenesis
       of AIDS. We have studied whether active CMV infection alters the cell
       tropism of HIV-1 in dually-infected individuals. Urines from
       HIV-seropositive individuals excreting CMV were compared to urines from
       CMV non-excretors. Sixty-six urines from HIV-seropositive individuals
       were tested. Infectious HIV-1 was not detected in any of the
       concentrated urines tested. The urines were filtered, concentrated,
       DNase-treated and cultured on HIV-1 non-permissive human forestin
       fibroblasts. HIV-1 DNA was detected by PCR with pol gene primers in 5 of
       39 MRHF cell cultures inoculated with CMV culture positive urine (p =
       0.037). HIV-1 DNA was not detected by PCR in uninfected fibroblasts, in
       fibroblasts inoculated with CMV uninfected urine from 27
       HIV-seropositive patients or in fibroblasts cultured with 9 CMV culture
       positive urines from 16 HIV-seronegative renal transplant recipients.
       Supernatant fluid from an HIV-1 PCR-positive culture was passaged onto
       another fibroblast monolayer, and these cells were negative for HIV-1
       DNA. Direct inoculation of fibroblasts with HIV-1 did not yield evidence
       of infection by PCR. CMV infection may facilitate HIV-1 DNA entry into
       ordinarily non-permissive cells.
 DE    Cells, Cultured  Cytomegalovirus Infections/*URINE  DNA, Viral/*ANALYSIS
       Fibroblasts/VIROLOGY  Human  HIV Seropositivity/*VIROLOGY
       HIV-1/*GENETICS  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).