Document 0276
 DOCN  M95A0276
 TI    Response of the regional lymph node to bluetongue virus infection in
       calves.
 DT    9510
 AU    Barratt-Boyes SM; Rossitto PV; Taylor BC; Ellis JA; MacLachlan NJ;
       Department of Veterinary Pathology, Microbiology and Immunology,; School
       of Veterinary Medicine, University of California, Davis; 95616, USA.
 SO    Vet Immunol Immunopathol. 1995 Mar;45(1-2):73-84. Unique Identifier :
       AIDSLINE MED/95328254
 AB    Bluetongue virus (BTV) infection of cattle is characterized by prolonged
       cell-associated viremia. In an effort to further evaluate the antiviral
       response of BTV-infected cattle, the role of the regional lymph node
       (LN) in the immune response of calves to BTV was characterized. Calves
       were inoculated with BTV in the skin of the neck in an area drained by
       the superficial cervical LN. Calves were euthanized at regular intervals
       after inoculation and both BTV-challenged and contralateral (control)
       superficial cervical LNs were harvested. In addition, some calves had
       cannulation of the superficial cervical efferent lymphatics prior to
       inoculation. Lymphocyte subpopulation analysis was done on LN cell
       suspensions and lymph cells using a panel of cell-specific monoclonal
       antibodies. There was a significant increase in the proportion of B
       cells in the challenged LN after inoculation as compared with the
       control LN. In addition, BTV-specific antibodies were detected in
       efferent lymph plasma from the challenged LN in one cannulated calf by 9
       days after inoculation (DAI), as determined by competitive enzyme-linked
       immunosorbent assay, whereas BTV-specific antibodies were not detected
       in serum from this calf through 12 DAI. Analysis of lymph cells revealed
       a sustained increase in cell output from the challenged LN due to an
       increase in lymphoblasts and CD8+ T cells. In contrast, the cell output
       from the control LN dropped markedly by 8 DAI and there was no
       significant increase in any specific cell population. Double label
       analysis characterized lymphoblasts as activated CD8+ cells, as
       determined by expression of MHC Class II antigens (CD8+ MHC II+). These
       cells were only transiently present as CD8+ MHC II+ cells were not
       identified in lymph from the challenged LN at 14 DAI. Few CD8+ MHC II+
       cells were identified at any time in lymph from the control LN or in
       lymph from a mock infected calf. The data indicate that B cell
       proliferation in the challenged LN and release of activated CD8+ cells
       from this LN were specific responses to BTV infection. The rapid
       expansion of activated CD8+ T cells indicates that these cells may limit
       early viral spread. It is concluded that the regional LN draining
       inoculated skin is critical to the immune response of calves to BTV
       infection.
 DE    Animal  Antibodies, Monoclonal  Antibodies, Viral/ANALYSIS
       B-Lymphocytes/IMMUNOLOGY  Bluetongue/*IMMUNOLOGY  Bluetongue
       Virus/*IMMUNOLOGY  Cattle  Cattle Diseases/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Enzyme-Linked Immunosorbent Assay/VETERINARY
       Flow Cytometry/VETERINARY  Lymph/IMMUNOLOGY  Lymph Nodes/*IMMUNOLOGY
       Lymphocyte Transformation  Male  Neck  Support, U.S. Gov't, Non-P.H.S.
       JOURNAL ARTICLE

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