Document 0277
 DOCN  M95A0277
 TI    Functional and phenotypic analysis of in vitro stimulated canine
       peripheral blood mononuclear cells.
 DT    9510
 AU    Rivas AL; Kimball ES; Quimby FW; Gebhard D; Department of Pathology, New
       York State College of Veterinary; Medicine, Cornell University, Ithaca
       14850, USA.
 SO    Vet Immunol Immunopathol. 1995 Mar;45(1-2):55-71. Unique Identifier :
       AIDSLINE MED/95328253
 AB    The inter-species cross-reactivity of cytokine bioassays for
       interleukin-2 (IL2) and interleukin-6 (IL6) was investigated in the
       canine species. The kinetics of normal canine peripheral blood
       mononuclear cells (PBMC) stimulated with pokeweed mitogen (PWM), were
       analyzed in terms of cytokine release and responsiveness to cytokine
       stimulation, in conjunction with determination of cell proliferation, de
       novo antibody synthesis and cell surface phenotype. PBMC were stimulated
       with PWM at the beginning of the culture and human recombinant IL2
       (rIL2) was added 3-4 days post stimulation (d.p.s.). Mitogenically
       stimulated cells proliferated and synthesized antibody in a linear
       fashion up to 6 d.p.s. Resting PBMC had a mean CD4+/CD8+ ratio of 1.7:1;
       whereas cells stimulated with PWM were predominantly of CD8 phenotype at
       7 d.p.s.. Three days after addition of IL2, stimulated cells were
       predominantly of the Thy+, sIg-, CD4+, CD8- phenotype, with an increase
       in the CD4+/CD8+ ratio. The magnitude of de novo antibody synthesis was
       lower in rIL2-supplemented cultures than in cultures stimulated only
       with PWM, and suggested a negative relationship between de novo antibody
       synthesis and proliferative responses of the same cultures. Supernatants
       from mitogen-stimulated cultures induced proliferation of mouse IL2- and
       IL6-dependent cell lines. Antibodies reactive with human IL2 or IL6
       inhibited these responses. IL2-like activity in PWM-stimulated culture
       peaked by 2 d.p.s. and decreased thereafter. IL6-like activity peaked
       later (4-6 d.p.s.).
 DE    Animal  B-Lymphocytes/*PHYSIOLOGY  Cell Differentiation  Cell Line
       Cells, Cultured  CD4-CD8 Ratio/VETERINARY  CD4-Positive
       T-Lymphocytes/*PHYSIOLOGY  CD8-Positive T-Lymphocytes/*PHYSIOLOGY
       Dogs/*IMMUNOLOGY  IgG/BIOSYNTHESIS  Immunophenotyping/*VETERINARY
       Interleukin-2/BIOSYNTHESIS/PHARMACOLOGY
       Interleukin-6/BIOSYNTHESIS/PHARMACOLOGY  Lymphocyte Transformation/DRUG
       EFFECTS/*PHYSIOLOGY  Pokeweed Mitogens/PHARMACOLOGY  Recombinant
       Proteins/PHARMACOLOGY  JOURNAL ARTICLE

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