Document 0278
 DOCN  M95A0278
 TI    Monocyte function in cattle experimentally infected with bovine
       immunodeficiency-like virus.
 DT    9510
 AU    Rovid AH; Carpenter S; Roth JA; Department of Microbiology, Immunology,
       and Preventive Medicine,; Iowa State University, Ames 50011, USA.
 SO    Vet Immunol Immunopathol. 1995 Mar;45(1-2):31-43. Unique Identifier :
       AIDSLINE MED/95328251
 AB    The effects of bovine immunodeficiency-like virus (BIV) on monocyte
       function were examined in experimentally infected cattle and in
       monocytes infected in vitro. Infection with the R29 isolate of BIV
       appeared to have relatively little effect on monocyte function in cattle
       during the first 2 years postinfection (PI). For the first 4 to 8 months
       post infection, monocyte phagocytosis of Staphylococcus aureus tended to
       be lower (P = 0.06) in BIV infected calves than in control animals.
       After 8 months PI, however, phagocytosis became equal between the two
       groups. Random and chemotactic migration and antibody-dependent
       cell-mediated cytotoxicity (ADCC) did not appear to be affected by BIV
       infection. Monocytes from BIV infected cattle were able to respond to in
       vitro treatment with interferon gamma similarly to monocytes from
       control cattle. Although experimental infection with BIV R29 resulted in
       minimal effects on monocyte function, this result could have been due
       either to a low virus burden in vivo or because BIV is intrinsically
       unable to affect monocyte function. To distinguish between these
       possibilities, monocytes from control, uninfected cattle were treated
       with BIV virus in vitro. Treatment of normal monocytes with cell-free
       virus significantly (P < 0.05) increased phagocytosis and random and
       chemotactic migration and decreased ADCC, in a dose-dependent manner. It
       appears, therefore, that the normal function of peripheral blood
       monocytes in the BIV R29 infected animals may be due to a low virus
       burden rather than to the inability of BIV to affect monocyte function.
       The in vitro infection results also raise the possibility that the
       function of monocyte derived cells at local sites of BIV replication may
       be altered.
 DE    Animal  Antibody-Dependent Cell Cytotoxicity/PHYSIOLOGY  Cattle  Cattle
       Diseases/IMMUNOLOGY/*PHYSIOPATHOLOGY  Chemotaxis, Leukocyte/PHYSIOLOGY
       Cytotoxicity Tests, Immunologic/VETERINARY  Immunodeficiency Virus,
       Bovine/ISOLATION & PURIF/*PHYSIOLOGY  Interferon-gamma,
       Recombinant/PHARMACOLOGY  Lentivirus
       Infections/IMMUNOLOGY/PHYSIOPATHOLOGY/*VETERINARY  Male  Monocytes/DRUG
       EFFECTS/*PHYSIOLOGY/VIROLOGY  Phagocytosis/PHYSIOLOGY  Support, U.S.
       Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.  Virus Replication
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).