Document 0288
 DOCN  M95A0288
 TI    Persistence of immunoglobulin heavy chain/c-myc recombination-positive
       lymphocyte clones in the blood of human immunodeficiency virus-infected
       homosexual men.
 DT    9510
 AU    Muller JR; Janz S; Goedert JJ; Potter M; Rabkin CS; Laboratory of
       Genetics, National Cancer Institute, National; Institutes of Health,
       Bethesda, MD 20892, USA.
 SO    Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6577-81. Unique Identifier :
       AIDSLINE MED/95327690
 AB    We studied blood lymphocytes of human immunodeficiency virus
       (HIV)-seropositive and -negative homosexual men for the presence of
       T(8;14) translocations that recombine c-myc and immunoglobulin
       heavy-chain (IgH) mu/IgH alpha switch regions. Clones with T(8;14)
       translocations were detected in 10.5% (12/114) of the HIV-positive and
       in 2.0% of the 99 uninfected patients. The majority of recombinations
       were found at a single time point only. Four patients, however, harbored
       multiple (up to four) and persistent (up to 9 years)
       translocation-positive cell clones. No correlation between the presence
       of these aberrant lymphocytes and a later lymphoma could be established.
       The exon 1/intron 1 region of the recombined c-myc was investigated for
       the presence of point mutations and these were found in the
       nonpersistent clones. Additional alterations detected in these clones
       included duplications and a deletion in the c-myc gene. The pattern of
       base substitution indicates that they were introduced after the
       translocation event.
 DE    Base Sequence  Chromosome Mapping  *Chromosomes, Human, Pair 14
       *Chromosomes, Human, Pair 8  Clone Cells  Cohort Studies  Comparative
       Study  DNA Primers  *Genes, myc  Homosexuality, Male  Human  HIV
       Seronegativity/*GENETICS/IMMUNOLOGY  HIV
       Seropositivity/*GENETICS/IMMUNOLOGY  Immunoglobulins,
       Heavy-Chain/BLOOD/*GENETICS  Lymphocytes/*IMMUNOLOGY/*PHYSIOLOGY  Male
       Molecular Sequence Data  Polymerase Chain Reaction  Recombination,
       Genetic  Restriction Mapping  *Translocation (Genetics)  JOURNAL ARTICLE

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