Document 0603
 DOCN  M95A0603
 TI    Routine immunizations of HIV-infected individuals and HIV replication.
       American Pediatric Society 104th annual meeting and Society for
       Pediatric Research 63rd annual meeting; 1994 May 2-5; Seattle.
 DT    9510
 AU    Ramilo O; Bell KD; Jue LS; Squires JE; Vitetta ES; UT Southwestern
       Medical Center, Dallas, USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):321 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330441
 AB    Two different populations of HIV-infected T cells are present in the
       peripheral blood of HIV-infected individuals: activated cells that
       produce virus and spread the infection, and quiescent cells that do not
       produce virus but harbor the viral genome. In vitro experiments have
       demonstrated that these quiescent cells are unable to produce virus
       unless they are activated, at which time the virus is transmitted to
       other cells. It has been hypothesized that immune stimulation derived
       from vaccinations could activate the quiescent HIV-infected cells and
       consequently enhance viral replication. This in turn could accelerate
       the course of the infection in HIV-infected individuals. To date, we
       have measured HIV titers by quantitative end-point dilution cultures of
       PBMC and plasma in 5 HIV-infected individuals who received influenza
       vaccine. Samples were obtained immediately before and 7-10 days after
       the administration of the vaccine. We observed an increased PBMC viral
       titer in 2 of 5 patients (10-fold and 100-fold respectively), but we did
       not detect any change in the plasma viral titer of samples obtained from
       those same individuals. In two patients we noted a 20-fold decrease in
       the titer of plasma virus after immunization. We are also measuring
       viral RNA and DNA by quantitative-competitive polymerase chain reaction.
       The data from these studies and from additional immunized children will
       be presented.
 DE    Cells, Cultured  DNA, Viral/ANALYSIS  Human  HIV/ISOLATION &
       PURIF/*PHYSIOLOGY  HIV Infections/IMMUNOLOGY/*VIROLOGY  Influenza
       Vaccine/*IMMUNOLOGY  Leukocytes, Mononuclear/IMMUNOLOGY  Polymerase
       Chain Reaction  RNA, Viral/ANALYSIS  Viremia  *Virus Replication
       MEETING ABSTRACT  JOURNAL ARTICLE

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