Document 0604
 DOCN  M95A0604
 TI    Quantitative immunofluorescent measurements of surface receptors on
       immune subsets from HIV infected children. American Pediatric Society
       104th annual meeting and Society for Pediatric Research 63rd annual
       meeting; 1994 May 2-5; Seattle.
 DT    9510
 AU    Denny TN; Stein D; Ince L; Mui T; Dieudonne A; Oleske JM; Department of
       Pediatrics, New Jersey Medical School, Newark, USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):321 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330440
 AB    It is possible to determine the antibody binding Capacity (ABC) per cell
       by performing quantitative immunofluorescent flow cytometric analysis of
       immune subsets. This study evaluated the number of ABC for CD3, CD4,
       CD8, CD16, and CD19 on lymphocytes and for CD4 and HLA-DR on monocytes.
       Peripheral blood samples were available from 16 HIV children that were
       grouped and staged as follows: Group I-asymptomatic (mean age = 1.7
       yrs/n = 3); Group II-symptomatic (mean age = 2.4 yrs/n = 6); Group
       III-AIDS (mean age = 4.0 yrs/n = 3) and Group IV-long term survivors
       (mean age = 10.5 yrs/n = 4). Data is expressed as mean number +/- SD of
       ABC/cell for each disease group. ABC for lymphocyte markers were as
       follows: CD3 (I = 120,050 +/- 1586, II = 122,465 +/- 13864, III =
       101,845 +/- 2818, IV = 95,348 +/- 11018); CD4 (I = 50,302 +/- 4284, II =
       54,157 +/- 4323, III = 50,949 +/- 5166, IV = 55,124 +/- 5039); CD8 (I =
       192,205 +/- 4477, II = 200,395 +/- 22683, III = 200,820 +/- 34018, IV =
       182,990 +/- 20864); CD16 (I = 17,536 +/- 7504, II = 13,940 +/- 10362,
       III = 12,025 +/- 5908, IV = 10,988 +/- 3730) and CD19 (I = 28,105 +/-
       1814, II = 29,085 +/- 4516, III = 26,898 +/- 11407, IV = 23,693 +/-
       2950) Monocyte expression of CD4 yielded (I = 9,684 +/- 1146, II =
       11,991 +/- 2491, III = 11,030 +/- 1540, IV = 11212 +/- 1937) and HLA-DR
       was (I = 105,311 +/- 16316, II = 133,886 +/- 9265, III = 144,909 +/-
       22419, IV = not done). These studies suggest a down regulation of CD3,
       CD16 and CD19 on lymphocytes and an up regulation of CD4 and HLA-DR on
       monocytes with HIV disease progression. Studies are underway to evaluate
       the potential influence of age on the distribution of ABC/cell and
       correlation with disease progression.
 DE    Antigens, CD/*ANALYSIS  Child  Child, Preschool  *Flow Cytometry  Human
       HIV Infections/*IMMUNOLOGY  HLA-DR Antigens/ANALYSIS  Infant
       *Lymphocyte Subsets  Monocytes/IMMUNOLOGY  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  MEETING ABSTRACT  JOURNAL ARTICLE

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