Document 0612
 DOCN  M95A0612
 TI    CD8-mediated suppression of autologous HIV-1 replication in a 5 week old
       HIV-1 infected infant. American Pediatric Society 104th annual meeting
       and Society for Pediatric Research 63rd annual meeting; 1994 May 2-5;
       Seattle.
 DT    9510
 AU    Pollack H; Zhan MX; Papaevangelou V; Chen SH; Tao P; Krasinski K;
       Borkowsky W; Dept. of Peds., New York University Medical Center, NY
       10016,; USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):320 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330432
 AB    Classical cytotoxic T cell (CTL) assays have failed to demonstrate
       cell-mediated killing of HIV-1 in infants less than 6 months of age.
       Using an in vitro EBV-stimulated lymphocyte culture technique we have
       been able to detect evidence of CD8-mediated control of HIV-1 viral
       replication in a 5-week old HIV-1 infected infant. PBLs were isolated by
       density-gradient centrifugation. CD8+ lymphocytes were removed by
       immunomagnetic beads and purity verified by flow cytometry. Parallel
       cultures of PBLs containing equivalent numbers of CD4+ lymphocytes and B
       cells with and without CD8+ cells were set-up. EBV was added and culture
       supernantants were collected weekly for one month and assayed for p24
       antigen and HIV antibody by ELISA and compared. In the lymphocyte
       culture depleted of CD8+ cells, p24 antigen production was 25 times
       higher (868 vs 32 pg/ml) than in the culture containing CD8+ cells. No
       effect of CD16 depletion on viral inhibition was seen. HIV-1 specific
       antibody production was detected in neither culture. In conclusion,
       cell-mediated immunity to autologous HIV-1 can be detected at a very
       early age in perinatally HIV-1 infected infants. Viral replication is
       inhibited by a CD8+, non-NK, T cell. Suppression of viral replication is
       observed before specific antibody production to HIV-1 can be detected.
       This non-CTL response may play an important role in the early control of
       viral replication in very young infants.
 DE    Cells, Cultured  CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Herpesvirus 4,
       Human  Human  HIV Antibodies/METABOLISM  HIV Core Protein p24/METABOLISM
       HIV Infections/*IMMUNOLOGY/VIROLOGY  HIV-1/IMMUNOLOGY/*PHYSIOLOGY  In
       Vitro  Infant  *Virus Replication  MEETING ABSTRACT  JOURNAL ARTICLE

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