Document 0613
 DOCN  M95A0613
 TI    Comparative evaluation of virus load in HIV-1 infected children showing
       slow and rapid disease progression by QC PCR. American Pediatric Society
       104th annual meeting and Society for Pediatric Research 63rd annual
       meeting; 1994 May 2-5; Seattle.
 DT    9510
 AU    Tetali S; Goonewardena H; Bakshi S; Miranda L; Pahwa S; Dept of Peds.,
       North Shore University Hospital-Cornell University; Medical College,
       Manhasset, NY, USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):320 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330431
 AB    This study is investigating virus load as evaluated by quantitative
       competitive PCR (QC PCR) in the peripheral blood mononuclear cells
       (PBMC) of children with HIV-1 infection. Initially we have examined 13
       children. Of these 6 were > 5 yrs (5-9 yrs), in CDC class P1, P2A or P2F
       and were designated as slow progressors (group 1). 7 were < or = 2 yrs
       (ages 0.3-2.0 yrs) and had developed symptoms at age < or = 1 yr,
       including 5 with AIDS defining illnesses and were designated rapid
       progressors (group 2). CD4 counts in grp 1 were > or = 20%, absolute >
       500 c/mm. In grp 2, CD4 counts met PCP prophylaxis guidelines in all but
       1 patient who developed PCP at age 0.6 months. DNA extracted from
       patient PBMC was analysed by PCR initially using cold nucleotides and
       subsequently by incorporating one hot nucleotide (alpha 32P dCTP) along
       with cold nucleotides in the reaction. The amplified DNA was quantitated
       for proviral copy number either by densitometry or by a phosphoimager.
       The copy numbers in grps 1&2 were different: grp 1, < or = 10,000, mean
       7,316; grp 2 > or = 10,000, mean 59,454 (p < 0.02 for mean values). This
       study suggests that virus load estimated by QC PCR is much higher in
       rapid progressors but its value in predicting rate of disease
       progression needs further evaluation. The study also indicates that QC
       PCR estimation of virus burden may permit sensitive and reliable
       quantitation of virus load.
 DE    Child  Child, Preschool  Comparative Study  CD4 Lymphocyte Count  Human
       HIV/ISOLATION & PURIF  HIV Infections/*DIAGNOSIS/IMMUNOLOGY/VIROLOGY
       *HIV-1  Polymerase Chain Reaction  Support, U.S. Gov't, P.H.S.  Time
       Factors  MEETING ABSTRACT  JOURNAL ARTICLE

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