Document 0615
 DOCN  M95A0615
 TI    The macrophage mannose receptor can mediate the uptake of Mycobacterium
       avium-intracellulare. American Pediatric Society 104th annual meeting
       and Society for Pediatric Research 63rd annual meeting; 1994 May 2-5;
       Seattle.
 DT    9510
 AU    Kruskal BA; Super M; Rogers R; Ezekowitz RA; Harvard Medical School,
       Children's Hospital, Div. of Infectious; Diseases, Boston, MA, USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):319 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330429
 AB    In patients with HIV and disseminated M. avium-intracellulare (MAI)
       infection, large numbers of MAI reside within macrophage phagosomes and
       survive there, resisting intrinsic macrophage antimycobacterial
       mechanisms. How MAI enter macrophages and survive has not been well
       elucidated thus far. Several macrophage cell-surface proteins have been
       shown to bind MAI, but none has yet been demonstrated to mediate uptake.
       Mycobacteria possess cell-membrane glycolipids rich in mannose, termed
       lipoarabinomannans, which suggested that the macrophage mannose receptor
       (MR), a phagocytic receptor, might be responsible for ingestion of MAI.
       We stably transfected HeLa cells with the cDNA for the human macrophage
       mannose receptor using the calcium phosphate method. Expressing cells
       were cloned twice. Stable HeLa transfectants expressing the MR were able
       to ingest MAI, as demonstrated by confocal fluorescence microscopy.
       Interestingly, non-transfected HeLa cells had a low level of intrinsic
       uptake activity. This observation demonstrates that the mannose receptor
       is able to mediate uptake of MAI, and that it may be important in the
       pathogenesis of MAI infection in AIDS. This experimental system will
       permit the comparison of different receptors for their ability to ingest
       MAI.
 DE    AIDS-Related Opportunistic Infections/METABOLISM/*MICROBIOLOGY  Child
       Hela Cells  Human  Macrophages/IMMUNOLOGY/METABOLISM/*MICROBIOLOGY
       Mycobacterium avium-intracellulare Infection/METABOLISM/  *MICROBIOLOGY
       Phagocytosis  Receptors, Cell Surface/*METABOLISM  Transfection  MEETING
       ABSTRACT  JOURNAL ARTICLE

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