Document 0620
 DOCN  M95A0620
 TI    Early determination of HIV infection status among infants born to HIV
       seropositive mothers. American Pediatric Society 104th annual meeting
       and Society for Pediatric Research 63rd annual meeting; 1994 May 2-5;
       Seattle.
 DT    9510
 AU    Paul M; Tetali S; Wang X; Bakshi S; Lesser M; Abrams E; Bamji M; Pahwa
       S; Dept of Peds, NSUH-CUMC, Manhasset, NY, USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):319 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330424
 AB    Early serologic diagnosis of HIV infection among infants born to HIV
       positive mothers is problematic because of transfer of maternal
       antibodies. Based on assay results of peripheral blood from 183 such
       infants, we formulated 4 sets of diagnostic decision rules incorporating
       individual assays as well as various assay combinations, to determine
       the HIV infection status of these children. Specimens were obtained
       within 1 month of birth and at 2-3 month intervals thereafter. The known
       clinical outcome was used as reference (CDC Class P2, 51 infants,
       seroreverters, 132 infants). Assays examined more than 600 specimens,
       and included: PCR, PBMC culture, p24 antigen determination and in vitro
       antibody production (IVAP). The most economical, convenient and
       sensitive algorithm, defined an infected patient as one with two
       consecutive PCR positive specimens, with the second also positive by
       culture. Two consecutive negative PCR specimens, with the second taken
       when the child is at least 3 months old, denoted an uninfected child. By
       this method we have identified 80% and 95% of infected, and 90% of
       uninfected children by ages 3 and 6 months, respectively.
 DE    Disease Transmission, Vertical  Female  Human  HIV
       Antibodies/BIOSYNTHESIS  HIV Core Protein p24/ANALYSIS  HIV
       Infections/*DIAGNOSIS/TRANSMISSION  *HIV Seropositivity  Infant  Infant,
       Newborn  Leukocytes, Mononuclear/IMMUNOLOGY  Polymerase Chain Reaction
       Pregnancy  *Pregnancy Complications, Infectious  Time Factors  MEETING
       ABSTRACT  JOURNAL ARTICLE

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