Document 0639
 DOCN  M95A0639
 TI    Induction of hematopoietic cytokines by HIV gp160 in cord blood.
       American Pediatric Society 104th annual meeting and Society for
       Pediatric Research 63rd annual meeting; 1994 May 2-5; Seattle.
 DT    9510
 AU    Than S; Oyaizu N; Pahwa S; North Shore University Hospital-Cornell
       University Medical; College, Department of Pediatrics, Manhasset, NY,
       USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):315 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330405
 AB    Aberrant immune activation and cytokine dysregulation occurs in HIV
       infection. Previously we have reported that HIV envelope protein gp160
       can enhance myelopoiesis in cord blood (Blood, 80: 1463, 1992). This
       study investigates influence of HIV gp160 in secretion of humoral CSFs:
       IL-3, IL-6 and GM-CSF which are essential for myelopoiesis, in cord
       blood. Whole cord blood (WCB) cells and purified cord blood T cells
       (CB-T) were treated with gp160 for 6 hours for detecting mRNA expression
       by RT-PCR and 24 hours for quantitation of cytokines by ELISA. All 3
       CSFs were induced in CB-T maximally with significantly lower amounts in
       WCB cells. Treatment with sCD4 consistently abolished IL-3 and GM-CSF
       production from both purified CB-T and WCB cells whereas IL-6 secretion
       was inhibited only in CB-T but enhanced in WCB. Our studies indicate
       that i) gp160 is a potent inducer of humoral CSFs in cord blood ii) that
       the major source of these cytokines in cord blood is T cells rather than
       monocytes and iii) induction of these cytokines is mediated through the
       interaction of gp160 with CD4 molecules but a different mechanism may be
       involved for IL-6 secretion from CB-non T cells.
 DE    Cytokines/*BIOSYNTHESIS  Enzyme-Linked Immunosorbent Assay  Fetal
       Blood/*CYTOLOGY  Gene Products, env/*PHARMACOLOGY
       Granulocyte-Macrophage Colony-Stimulating Factor/BIOSYNTHESIS  Human
       HIV  Interleukin-3/BIOSYNTHESIS  Interleukin-6/BIOSYNTHESIS  Polymerase
       Chain Reaction  Protein Precursors/*PHARMACOLOGY  Support, U.S. Gov't,
       P.H.S.  MEETING ABSTRACT  JOURNAL ARTICLE

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