Document 0640
 DOCN  M95A0640
 TI    Induction of cytokine expression in trophoblastoid JAR cells by immune
       complexes: implications for vertical transmission of HIV. American
       Pediatric Society 104th annual meeting and Society for Pediatric
       Research 63rd annual meeting; 1994 May 2-5; Seattle.
 DT    9510
 AU    Jarvis JN; Moore HT; Iobidze M; Dept. of Pediatrics, Wayne St. U.,
       Detroit, MI, USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):315 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330404
 AB    Mechanisms through which HIV is transferred from mother to fetus remain
       unknown. It has been speculated that infectious HIV immune complexes
       (IC's) may be the vector for viral to entry into fetal tissue. We
       propose another mechanism which may explain how IC's facilitate the
       spread of HIV from mother to fetus: the induction of cytokines which
       regulate the expression of HIV in CD4+ cells. We incubated model
       BSA-anti-BSA immune complexes with a human choriocarcinoma cell line
       (JAR cells) for 72 hours, after which levels of TNF alpha, IL-1 beta,
       and IL-6 were measured in cell supernates by ELISA. JAR cells do not
       constitutively express either TNF alpha or IL-1 beta. However, in the
       presence of immune complexes, IL-1 beta levels in cell supernates were
       277 +/- 67 pg/ml, and TNF alpha levels were 325 +/- 47 pg/ml. No
       detectable levels of either cytokine were observed when JAR cells were
       incubated with monomeric IgG. JAR cells constitutively express IL-6, and
       these levels were not increased when cells were incubated with immune
       complexes. Whether the initial HIV infection of placental tissue occurs
       via trophoblastic cells or CD4+ tissue macrophages, these data show that
       IC's binding to trophoblastic cells may induce cytokines (especially TNF
       alpha) which could then induce HIV expression in either maternal
       lymphocytes or previously-infected placental macrophages.
 DE    Antigen-Antibody Complex/*PHYSIOLOGY  Choriocarcinoma/*METABOLISM
       Cytokines/*BIOSYNTHESIS  *Disease Transmission, Vertical  Human  HIV
       Infections/IMMUNOLOGY/*TRANSMISSION  Interleukin-1/BIOSYNTHESIS
       Interleukin-6/BIOSYNTHESIS  Tumor Cells, Cultured  Tumor Necrosis
       Factor/BIOSYNTHESIS  MEETING ABSTRACT  JOURNAL ARTICLE

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