Document 0646
 DOCN  M95A0646
 TI    Ontogeny of HIV-1 specific CTL in vertical infection. American Pediatric
       Society 104th annual meeting and Society for Pediatric Research 63rd
       annual meeting; 1994 May 2-5; Seattle.
 DT    9510
 AU    Holmes DR; Heerema AE; Sullivan JL; Luzuriaga K; Dept. of Pediatrics,
       Univ. of Massachusetts Medical School,; Worcester, USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):314 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330398
 AB    The capability of the young infant to mount virus-specific CTL responses
       is not well-described. We have previously described a deficiency of
       HIV-1 specific CTL in older HIV-1 infected children. HIV-1 specific CTL
       responses were studied in 11 infants born to HIV-1 seropositive women; 7
       of those infants were 4 months or younger at first study. Primary HIV-1
       specific CTL were detected in 5 of these infants within the first year
       of life: gag-specific CTL in 3 infants, env-specific CTL in 4 infants,
       and nef-specific CTL in 1 infant. Primary pol-specific CTL were not
       detected in any of the infants studied. Primary HIV-1 specific CTL were
       detected as early as 3 months of age in one infant, but were not
       detected until 8-11 months of age in 4 other infants. Using non-specific
       or virus-specific in vitro stimulation, secondary HIV-1 specific CTL
       were detected as early as 3 months of age in one infant who lacked
       primary CTL, but were not detected in other infants until the time when
       primary CTL activity was evident (10-11 months of age). These findings
       contrast with reports of the detection of primary and secondary HIV-1
       specific CTL in adults soon after acute primary infection. Primary and
       secondary HIV-1 specific responses were detected both in infants who
       experienced in utero infection as well as in infants who experienced
       intrapartum infection. Characterization of HIV-1 specific CTL lines
       (phenotype, epitope specificity, HLA restriction elements, and ability
       to control viral replication in vitro) generated from serial blood
       samples drawn from these infants is underway. Neither primary nor
       secondary HIV-1 specific CTL have been detected in the circulation of
       any uninfected children studied. Further examination of HIV-1 specific
       CTL, particularly in early infancy, may be important for the development
       of vaccine strategies to interrupt vertical transmission.
 DE    *Disease Transmission, Vertical  Human  HIV
       Infections/*IMMUNOLOGY/TRANSMISSION  HIV-1/*IMMUNOLOGY  Infant
       T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  MEETING ABSTRACT  JOURNAL ARTICLE

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