Document 0648
 DOCN  M95A0648
 TI    Effect of CD8 T cell lines derived from vertically HIV infected children
       on HIV replication in naturally infected cells. American Pediatric
       Society 104th annual meeting and Society for Pediatric Research 63rd
       annual meeting; 1994 May 2-5; Seattle.
 DT    9510
 AU    Heerema AE; Holmes DR; Sullivan JL; Luzuriaga K; Dept. of Pediatrics,
       Univ. of Mass. Medical Center, Worcester,; USA.
 SO    Pediatr AIDS HIV Infect. 1994 Oct;5(5):314 (unnumbered abstract). Unique
       Identifier : AIDSLINE AIDS/95330396
 AB    The CD8 T cell population undergoes rapid early expansion in vertical
       HIV infection, yet the functional capabilities of those CD8 T cells are
       not well defined. Our lab has previously described a deficient CD8 T
       cell mediated HIV-specific CTL response in vertically-infected children.
       Several groups have reported a specific anti-viral effect of CD8 T cells
       from HIV-infected adults. CD8 lymphocytes from these persons have been
       shown to inhibit viral replication in infected cell lines and PBMC. This
       phenomenon appears to be mediated by release of a soluble anti-viral
       factor by the CD8 T cells. We have begun to investigate the ability of
       CD8 T cells from vertically infected children to inhibit viral
       replication in autologous and heterologous naturally HIV-infected CD4 T
       cell lines. Cell lines were derived from vertically infected children
       ranging from 3 mos. to 7 yrs. of age, and on HIV-uninfected controls
       through stimulation of PBMC with PHA and IL-2, and subsequent selection
       for CD4 and CD8 fractions. HIV specific cytolytic potential of the CD8 T
       cell lines was assessed by combining them with autologous
       B-lymphoblastoid cell lines infected with vaccinia constructs expressing
       HIV-1 gene products. Cocultures were performed in triplicate in 96-well
       plates by combining 10(5) CD4 T cells per well with 0.1 x 10(5), 2 x
       10(5), or 5 x 10(5) CD8 T cells. Inhibition of viral replication was
       assessed by supernatant p24 Ag, in situ hybridization, and PCR. Viral
       replication was inhibited in a dose dependent manner by CD8 T cell lines
       derived from five children; degree of inhibition varied among lines. No
       significant HLA restriction was observed for this anti-viral effect, nor
       did cells from HIV-infected children differ in anti-viral capability
       from those of uninfected controls. CD8 T cell lines from one child
       appeared to augment viral replication despite high cytolytic
       capabilities in the CTL assay. Further studies to elucidate the
       mechanism of CD8 T cell inhibition of viral replication are underway.
 DE    Cell Line  Child  Child, Preschool  CD4-Positive
       T-Lymphocytes/PHYSIOLOGY  CD8-Positive T-Lymphocytes/*PHYSIOLOGY
       *Disease Transmission, Vertical  Human  HIV/*PHYSIOLOGY  HIV Core
       Protein p24/ANALYSIS  HIV Infections/*IMMUNOLOGY/TRANSMISSION/VIROLOGY
       In Situ Hybridization  Infant  Polymerase Chain Reaction  *Virus
       Replication  MEETING ABSTRACT  JOURNAL ARTICLE

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